Abstract：
Maternal protein malnutrition during developmental periods might impair the redox state and the brain\'s excitatory/inhibitory neural network, increasing central sympathetic tone. Conversely, moderate physical exercise at an early age reduces the risk of chronic diseases. Thus, we hypothesized that a moderate training protocol could reduce the harmful effects of a low-protein maternal diet on the brainstem of young male offspring. We used a rat model of maternal protein restriction during the gestational and lactation period followed by an offspring\'s continuous treadmill exercise. Pregnant rats were divided into two groups according to the protein content in the diet: normoprotein (NP), receiving 17% of casein, and low protein (LP), receiving 8% of casein until the end of lactation. At 30 days of age, the male offspring were further subdivided into sedentary (NP-Sed and LP-Sed) or exercised (NP-Ex and LP-Ex) groups. Treadmill exercise was performed as follows: 4 weeks, 5 days/week, 60 min/day at 50% of maximal running capacity. The trained animals performed a treadmill exercise at 50% of the maximal running capacity, 60 min/day, 5 days/week, for 4 weeks. Our results indicate that a low-protein diet promotes deficits in the antioxidant system and a likely mitochondrial uncoupling. On the other hand, physical exercise restores the redox balance, which leads to decreased oxidative stress caused by the diet. In addition, it also promotes benefits to GABAergic inhibitory signaling. We conclude that regular moderate physical exercise performed in youthhood protects the brainstem against changes induced by maternal protein restriction.
摘要：
在发育期母体蛋白质营养不良可能会损害REDOX状态和大脑的兴奋性/抑制性神经网络，增加中枢交感神经张力。相反，在幼年时期进行适度的体育锻炼可以降低患慢性病的风险。因此，我们假设适度的训练方案可以减少低蛋白母体饮食对年轻男性后代脑干的有害影响.我们在妊娠期和哺乳期使用了母体蛋白质限制的大鼠模型，然后进行后代的连续跑步机运动。妊娠大鼠根据饮食中的蛋白质含量分为两组：正常蛋白（NP），接受17%的酪蛋白，低蛋白（LP），接受8%的酪蛋白直到哺乳期结束。在30天大的时候，将雄性后代进一步细分为久坐（NP-Sed和LP-sed）或运动（NP-Ex和LP-Ex）组。跑步机运动如下：4周，5天/周,60分钟/天，50％的最大运行能力。受过训练的动物以最大跑步能力的50％进行跑步机锻炼，60分钟/天,5天/周,4周。我们的结果表明，低蛋白饮食会促进抗氧化系统的缺陷和可能的线粒体解偶联。另一方面,体育锻炼可以恢复REDOX平衡，从而减少饮食引起的氧化应激。此外,它还可以促进GABA能抑制信号的益处。我们得出的结论是，在青年时期进行定期适度的体育锻炼可以保护脑干免受母体蛋白质限制引起的变化。
