Abstract：
Panax quinquefolius is a natural homology medicine and food that is rich in bioactive ingredients, such as ginsenosides and polysaccharides. The combination of ultra-performance liquid chromatography quadrupole/time of flight-mass spectrometry (UPLC-Q-TOF/MS) and desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was used for the first time in a spatial metabolomics analysis to comprehensively evaluate the differential components in different microregions of P. quinquefolius. UPLC-Q-TOF/MS and DESI-MSI combined with principal component analysis and orthogonal partial least squares-discriminant analysis were used to screen differential metabolites. UPLC-Q-TOF/MS and DESI-MSI screened 27 and 23 differential metabolites, respectively, among which 15 differential metabolites were identified by both methods. It was found that some components, such as ginsenoside Rg1 and malonyl-ginsenoside Rc, were mainly distributed in P of the transverse slice of P. quinquefolius roots, while ginsenoside Ro and malonyl-ginsenoside Rd were mainly distributed in C. The methods and results of this study could be used to understand the precise localization, biosynthesis, and biological functions of special metabolites in P. quinquefolius.
摘要：
西洋参是一种富含生物活性成分的天然同源药物和食品，如人参皂苷和多糖。首次将超高效液相色谱-四极杆/飞行时间-质谱(UPLC-Q-TOF/MS)和解吸电喷雾质谱成像(DESI-MSI)相结合用于空间代谢组学分析,全面评价了西洋参不同微区的差异成分。采用UPLC-Q-TOF/MS和DESI-MSI结合主成分分析和正交偏最小二乘-判别分析筛选差异代谢产物。UPLC-Q-TOF/MS和DESI-MSI共筛选出27种和23种差异代谢物,分别,其中通过两种方法鉴定了15种差异代谢物。发现一些组件，如人参皂苷Rg1和丙二酰人参皂苷Rc，主要分布在西洋参根横切面的P中，而人参皂苷Ro和丙二酰人参皂苷Rd主要分布在C中。本研究的方法和结果可用于理解精确定位，生物合成，和西洋参特殊代谢产物的生物学功能。
