PDF(Pubmed)
Abstract：
Southern corn rust (SCR) caused by Puccinia polysora Underw is a major disease leading to severe yield losses in China Summer Corn Belt. Using six multi-locus GWAS methods, we identified a set of SCR resistance QTNs from a diversity panel of 140 inbred lines collected from China Summer Corn Belt. Thirteen QTNs on chromosomes 1, 2, 4, 5, 6, and 8 were grouped into three types of allele effects and their associations with SCR phenotypes were verified by post-GWAS case-control sampling, allele/haplotype effect analysis. Relative resistance (RRR) and relative susceptibility (RRs) catering to its inbred carrier were estimated from single QTN and QTN-QTN combos and epistatitic effects were estimated for QTN-QTN combos. By transcriptomic annotation, a set of candidate genes were predicted to be involved in transcriptional regulation (S5_145, Zm00001d01613, transcription factor GTE4), phosphorylation (S8_123, Zm00001d010672, Pgk2- phosphoglycerate kinase 2), and temperature stress response (S6_164a/S6_164b, Zm00001d038806, hsp101, and S5_211, Zm00001d017978, cellulase25). The breeding implications of the above findings were discussed.
摘要：
由PucciniapolysoraUnderw引起的南方玉米锈病（SCR）是导致中国夏玉米带严重产量损失的主要疾病。使用六种多位点GWAS方法，我们从中国夏玉米带收集的140个自交系的多样性小组中确定了一组SCR抗性QTNs。将染色体1、2、4、5、6和8上的13个QTNs分为三种类型的等位基因效应，并通过GWAS病例对照采样验证了它们与SCR表型的关联。等位基因/单倍型效应分析。从单个QTN和QTN-QTN组合中估计了适应其近交携带者的相对抗性（RRR）和相对敏感性（RR），并估计了QTN-QTN组合的认识论效应。通过转录组学注释，预测一组候选基因参与转录调控（S5_145，Zm00001d01613，转录因子GTE4），磷酸化（S8_123，Zm00001d010672，Pgk2-磷酸甘油酸激酶2），和温度胁迫响应(S6_164a/S6_164b，Zm00001d038806、hsp101和S5_211、Zm00001d017978、纤维素酶25)。讨论了上述发现的育种意义。
