PDF(Pubmed)
Abstract：
UNASSIGNED: The protozoan parasite Trichomonas vaginalis is the most common cellular sexually transmitted disease in humans, and the closely related species Trichomonas gallinae is an avian parasite of ecological and economic importance. Phylogenetic evidence suggests T. vaginalis arose during bird to human transmission of a T. gallinae-like ancestor. Trichomonas vaginalis shares a strong clinical association with the independent sexually transmitted pathogen Metamycoplasma (formerly Mycoplasma) hominis, and the uncultured bacterium \"Candidatus Malacoplasma (formerly Mycoplasma) girerdii,\" with the latter association being an order of magnitude stronger. Both bacterial species have been shown to profoundly influence T. vaginalis growth, energy production and virulence-associated mechanisms.
UNASSIGNED: Evidence for a novel Malacoplasma sp. was discovered by in vivo Illumina metatranscriptomics sequencing of the T. gallinae-infected pigeon mouth. We leveraged published 16S rDNA profiling data from digestive tract of 12 healthy and 24 T. gallinae-infected pigeons to investigate association between the novel Malacoplasma sp. and T. gallinae. We utilised Illumina metagenomics sequencing targeted to pigeon oral and crop samples infected with the novel Malacoplasma sp. to generate its full-length genome sequence. Sequence similarity network analysis was used to compare annotated proteins from the novel Malacoplasma sp. with a range of other related species.
UNASSIGNED: Here we present evidence for a novel Malacoplasma species, related to \"Ca. M. girerdii,\" that is strongly associated with T. gallinae in the upper digestive tract of domestic pigeons. Analysis of the genome sequence revealed gene features apparently specific to a Trichomonas-symbiotic Malacoplasma lineage.
UNASSIGNED: These data support a model of long-term association between Trichomonas and Malacoplasma spp. that has been conserved across diversification of the Trichomonas lineage and the host species barrier from birds to human.
UNASSIGNED: Evidence for a novel Malacoplasma sp. was discovered by in vivo Illumina metatranscriptomics sequencing of the T. gallinae-infected pigeon mouth. We leveraged published 16S rDNA profiling data from digestive tract of 12 healthy and 24 T. gallinae-infected pigeons to investigate association between the novel Malacoplasma sp. and T. gallinae. We utilised Illumina metagenomics sequencing targeted to pigeon oral and crop samples infected with the novel Malacoplasma sp. to generate its full-length genome sequence. Sequence similarity network analysis was used to compare annotated proteins from the novel Malacoplasma sp. with a range of other related species.
UNASSIGNED: Here we present evidence for a novel Malacoplasma species, related to \"Ca. M. girerdii,\" that is strongly associated with T. gallinae in the upper digestive tract of domestic pigeons. Analysis of the genome sequence revealed gene features apparently specific to a Trichomonas-symbiotic Malacoplasma lineage.
UNASSIGNED: These data support a model of long-term association between Trichomonas and Malacoplasma spp. that has been conserved across diversification of the Trichomonas lineage and the host species barrier from birds to human.
摘要：
■原虫寄生虫阴道毛滴虫是人类最常见的细胞性传播疾病，和密切相关的物种毛滴虫是具有生态和经济重要性的鸟类寄生虫。系统发育证据表明，阴道毛虫是在鸟类向人类传播类似于鸡毛虫的祖先过程中出现的。阴道毛滴虫与独立的性传播病原体人型支原体（以前的支原体）具有很强的临床相关性，和未培养的细菌“念珠菌Malacoplasma（以前的支原体）girerdii，“与后一种关联的数量级更强。两种细菌都被证明对阴道毛虫的生长有深远的影响，能量生产和毒力相关机制。
■新型疟原虫的证据。是通过体内Illumina转移转录组学测序发现的。我们利用已发表的来自12只健康和24只感染了鸡的鸽子的消化道的16SrDNA分析数据来研究新型的疟原虫之间的关联。还有T.gallinae.我们利用Illumina宏基因组学测序技术针对感染了新型疟原虫的鸽子口腔和作物样品。生成其全长基因组序列。序列相似性网络分析用于比较来自新型疟原虫的注释蛋白质。与一系列其他相关物种。
■在这里，我们提供了一种新的疟原虫物种的证据，与“Ca”有关。M.girerdii,这与家鸽上消化道中的鸡毛虫密切相关。对基因组序列的分析揭示了毛滴虫共生的疟原虫谱系显然具有特异性的基因特征。
■这些数据支持毛滴虫和疟原虫之间的长期关联模型。在毛滴虫谱系的多样化和从鸟类到人类的宿主物种屏障中得到了保护。
■新型疟原虫的证据。是通过体内Illumina转移转录组学测序发现的。我们利用已发表的来自12只健康和24只感染了鸡的鸽子的消化道的16SrDNA分析数据来研究新型的疟原虫之间的关联。还有T.gallinae.我们利用Illumina宏基因组学测序技术针对感染了新型疟原虫的鸽子口腔和作物样品。生成其全长基因组序列。序列相似性网络分析用于比较来自新型疟原虫的注释蛋白质。与一系列其他相关物种。
■在这里，我们提供了一种新的疟原虫物种的证据，与“Ca”有关。M.girerdii,这与家鸽上消化道中的鸡毛虫密切相关。对基因组序列的分析揭示了毛滴虫共生的疟原虫谱系显然具有特异性的基因特征。
■这些数据支持毛滴虫和疟原虫之间的长期关联模型。在毛滴虫谱系的多样化和从鸟类到人类的宿主物种屏障中得到了保护。
