Abstract：
Cardiovascular diseases are the leading causes of mortality and morbidity worldwide. Atherosclerotic plaque underlies the predominant factors and is composed of various cell types, including structure cells, such as endothelial and smooth muscle cells, and immune cells, such as macrophages and T cells. Single-cell RNA sequencing (scRNA-seq) has been extensively applied to decipher these cellular heterogeneities to expand our understanding on the mechanisms of atherosclerosis (AS) and to facilitate identifying cell-type-specific long noncoding RNAs (LncRNAs). LncRNAs have been demonstrated to deeply regulate biological activities at the transcriptional and post-transcriptional levels. A group of well-documented functional lncRNAs in AS have been studied. In our review, we selectively described several lncRNAs involved in the critical process of AS. We highlighted four novel lncRNAs (lncRNA CARMN, LINC00607, PCAT19, LINC01235) detected in scRNA-seq datasets and their functions in AS. We also reviewed open web source and bioinformatic tools, as well as the latest methods to perform an in-depth study of lncRNAs. It is fundamental to annotate functional lncRNAs in the various biological activities of AS, as lncRNAs may represent promising targets in the future for treatment and diagnosis in clinical practice.
摘要：
心血管疾病是全世界死亡率和发病率的主要原因。动脉粥样硬化斑块是主要因素的基础，由各种细胞类型组成，包括结构细胞，如内皮细胞和平滑肌细胞，和免疫细胞，如巨噬细胞和T细胞。单细胞RNA测序（scRNA-seq）已被广泛应用于破译这些细胞异质性，以扩大我们对动脉粥样硬化（AS）机制的理解，并促进识别细胞类型特异性长链非编码RNA（LncRNA）。LncRNAs已被证明在转录和转录后水平上深度调节生物活性。已经研究了AS中一组有据可查的功能性lncRNAs。在我们的审查中,我们选择性地描述了几种参与AS关键过程的lncRNAs。我们强调了四个新的lncRNA(lncRNACARMN，LINC00607，PCAT19，LINC01235）在scRNA-seq数据集中检测到及其在AS中的功能。我们还回顾了开源和生物信息学工具，以及对lncRNAs进行深入研究的最新方法。在AS的各种生物活性中注释功能lncRNAs是至关重要的，lncRNAs可能是未来临床实践中治疗和诊断的有希望的靶标。
