Abstract：
Leaf subepidermal secretory cavities are a notable trait in Myrtaceae, but their formation is still controversial because of the lack of consensus on their ontogeny among authors. Knowledge about the compounds present in these cavities has grown over the last few years, demonstrating that terpenoid-rich oils are not their unique content. These two points are the focus of this study on the ontogeny, structure, and contents of secretory cavities in neotropical Myrtaceae.
We used histochemical tests and Raman analysis to verify the basic chemical composition of the cavity contents of nine species. We studied the ontogeny of glands in one species, comparing aldehyde-fixed tissues and fresh sections mounted in an inert medium.
We observed schizogenous development and appearance of the secretory cavities and found that sample processing may induce cell breakdown, which can be misinterpreted as lysigeny. The content of these cavities contains putative terpenes, resins, carbonyl groups, and flavonoids.
Our findings support the hypothesis that the lysigenous appearance of the oil glands is a technical artifact. These tissue distortions must be considered when interpreting the development of this type of secretory structure. Moreover, the basic analyses of chemical constituents show for the first time that the glands of neotropical Myrtaceae are potential reservoirs of some compounds such as flavonoids previously reported as novelties for a few other myrtaceous species. Because some of them are non-lipid compounds, the idea that the glands are just oil repositories is no longer applicable.
We used histochemical tests and Raman analysis to verify the basic chemical composition of the cavity contents of nine species. We studied the ontogeny of glands in one species, comparing aldehyde-fixed tissues and fresh sections mounted in an inert medium.
We observed schizogenous development and appearance of the secretory cavities and found that sample processing may induce cell breakdown, which can be misinterpreted as lysigeny. The content of these cavities contains putative terpenes, resins, carbonyl groups, and flavonoids.
Our findings support the hypothesis that the lysigenous appearance of the oil glands is a technical artifact. These tissue distortions must be considered when interpreting the development of this type of secretory structure. Moreover, the basic analyses of chemical constituents show for the first time that the glands of neotropical Myrtaceae are potential reservoirs of some compounds such as flavonoids previously reported as novelties for a few other myrtaceous species. Because some of them are non-lipid compounds, the idea that the glands are just oil repositories is no longer applicable.
摘要：
目的：叶表皮下分泌腔是桃金娘科的一个显著特征。由于作者对其个体发育缺乏共识，因此这些分泌结构的形成仍然存在争议。在过去的几年中，关于这些腔中存在的化合物的性质的知识已经增长，证明富含萜类化合物的油不是它们独特的成分。这两点是本文研究的重点,调查个体发育，新热带桃金娘科分泌腔的结构和含量。
方法：我们使用组织化学测试和拉曼分析验证了9个物种的空洞含量的基本化学成分，并研究了一个物种的腺体的个体发育，比较醛固定的组织和安装在惰性介质中的新鲜切片。
结果：我们观察了分泌腔中的分裂发育和外观，发现组织处理可能会诱导细胞分解，这可能会被误解为lysigeny。这些洞的内容含有预期的萜烯，树脂,羰基,和类黄酮.
结论：我们的研究结果支持以下假设：技术伪影可能会在油腺中产生溶解性印象。在解释这种分泌结构的发展时，必须考虑这些组织变形。此外，对化学成分的基本分析首次表明，新热带桃金娘科的腺体是一些化合物的潜在储库，这些化合物先前被报道为其他一些桃金娘物种的新颖性，如类黄酮。因为它们中的一些是非脂质化合物，腺体只是油库的想法不再适用。本文受版权保护。保留所有权利。
方法：我们使用组织化学测试和拉曼分析验证了9个物种的空洞含量的基本化学成分，并研究了一个物种的腺体的个体发育，比较醛固定的组织和安装在惰性介质中的新鲜切片。
结果：我们观察了分泌腔中的分裂发育和外观，发现组织处理可能会诱导细胞分解，这可能会被误解为lysigeny。这些洞的内容含有预期的萜烯，树脂,羰基,和类黄酮.
结论：我们的研究结果支持以下假设：技术伪影可能会在油腺中产生溶解性印象。在解释这种分泌结构的发展时，必须考虑这些组织变形。此外，对化学成分的基本分析首次表明，新热带桃金娘科的腺体是一些化合物的潜在储库，这些化合物先前被报道为其他一些桃金娘物种的新颖性，如类黄酮。因为它们中的一些是非脂质化合物，腺体只是油库的想法不再适用。本文受版权保护。保留所有权利。
