We used histochemical tests and Raman analysis to verify the basic chemical composition of the cavity contents of nine species. We studied the ontogeny of glands in one species, comparing aldehyde-fixed tissues and fresh sections mounted in an inert medium.
We observed schizogenous development and appearance of the secretory cavities and found that sample processing may induce cell breakdown, which can be misinterpreted as lysigeny. The content of these cavities contains putative terpenes, resins, carbonyl groups, and flavonoids.
Our findings support the hypothesis that the lysigenous appearance of the oil glands is a technical artifact. These tissue distortions must be considered when interpreting the development of this type of secretory structure. Moreover, the basic analyses of chemical constituents show for the first time that the glands of neotropical Myrtaceae are potential reservoirs of some compounds such as flavonoids previously reported as novelties for a few other myrtaceous species. Because some of them are non-lipid compounds, the idea that the glands are just oil repositories is no longer applicable.
方法:我们使用组织化学测试和拉曼分析验证了9个物种的空洞含量的基本化学成分,并研究了一个物种的腺体的个体发育,比较醛固定的组织和安装在惰性介质中的新鲜切片。
结果:我们观察了分泌腔中的分裂发育和外观,发现组织处理可能会诱导细胞分解,这可能会被误解为lysigeny。这些洞的内容含有预期的萜烯,树脂,羰基,和类黄酮.
结论:我们的研究结果支持以下假设:技术伪影可能会在油腺中产生溶解性印象。在解释这种分泌结构的发展时,必须考虑这些组织变形。此外,对化学成分的基本分析首次表明,新热带桃金娘科的腺体是一些化合物的潜在储库,这些化合物先前被报道为其他一些桃金娘物种的新颖性,如类黄酮。因为它们中的一些是非脂质化合物,腺体只是油库的想法不再适用。本文受版权保护。保留所有权利。