PDF(Pubmed)
Abstract：
Complex fibrillar networks mediate liquid-liquid phase separation of biomolecular condensates within the cell. Mechanical interactions between these condensates and the surrounding networks are increasingly implicated in the physiology of the condensates and yet, the physical principles underlying phase separation within intracellular media remain poorly understood. Here, we elucidate the dynamics and mechanics of liquid-liquid phase separation within fibrillar networks by condensing oil droplets within biopolymer gels. We find that condensates constrained within the network pore space grow in abrupt temporal bursts. The subsequent restructuring of condensates and concomitant network deformation is contingent on the fracture of network fibrils, which is determined by a competition between condensate capillarity and network strength. As a synthetic analog to intracellular phase separation, these results further our understanding of the mechanical interactions between biomolecular condensates and fibrillar networks in the cell.
摘要：
复杂的纤丝网络介导细胞内生物分子缩合物的液-液相分离。这些冷凝物与周围网络之间的机械相互作用越来越多地涉及冷凝物的生理学，细胞内介质内相分离的物理原理仍然知之甚少。这里,我们通过在生物聚合物凝胶中冷凝油滴来阐明纤维网络中液液相分离的动力学和力学。我们发现，约束在网络孔隙空间内的冷凝物在突然的时间爆发中增长。冷凝物的后续重组和伴随的网络变形取决于网络原纤维的断裂，这是由凝析油毛细作用和网络强度之间的竞争决定的。作为细胞内相分离的合成类似物，这些结果进一步理解了细胞内生物分子凝聚物和纤丝网络之间的机械相互作用.
