PDF(Pubmed)
Abstract：
The diversity of viruses identified from the various niches of the human oral cavity-from saliva to dental plaques to the surface of the tongue-has accelerated in the age of metagenomics. This rapid expansion demonstrates that our understanding of oral viral diversity is incomplete, with only a few studies utilizing passive drool collection in conjunction with metagenomic sequencing methods. For this pilot study, we obtained 14 samples from healthy staff members working at the Duke Lemur Center (Durham, NC, USA) to determine the viral diversity that can be identified in passive drool samples from humans. The complete genomes of 3 anelloviruses, 9 cressdnaviruses, 4 Caudoviricetes large bacteriophages, 29 microviruses, and 19 inoviruses were identified in this study using high-throughput sequencing and viral metagenomic workflows. The results presented here expand our understanding of the vertebrate-infecting and microbe-infecting viral diversity of the human oral virome in North Carolina (USA).
摘要：
在宏基因组学时代，从人类口腔的各种壁龛中鉴定出的病毒多样性-从唾液到牙斑再到舌头表面-已经加速。这种快速扩张表明我们对口服病毒多样性的理解是不完整的，只有少数研究利用被动滴液收集结合宏基因组测序方法。对于这项试点研究，我们从在杜克狐猴中心(达勒姆，NC,美国）确定可以在人类被动口水样品中鉴定的病毒多样性。3种软骨病毒的完整基因组，9个cressdnavirus,4Caudoviricetes大型噬菌体，29个微小病毒，在这项研究中，使用高通量测序和病毒宏基因组工作流程鉴定了19种病毒。此处提供的结果扩展了我们对北卡罗来纳州（美国）人类口腔病毒的脊椎动物感染和微生物感染病毒多样性的理解。
