PDF(Pubmed)
Abstract：
Aminoglycoside antimicrobials remain valuable therapeutic options, but their effectiveness has been threatened by the production of bacterial 16S ribosomal RNA methyltransferases (16S-RMTases). In this study, we evaluated the genomic epidemiology of 16S-RMTase genes among Gram-negative bacteria circulating in the American continent. A total of 4877 16S-RMTase sequences were identified mainly in Enterobacterales and nonfermenting Gram-negative bacilli isolated from humans, animals, foods, and the environment during 1931-2023. Most of the sequences identified were found in the United States, Brazil, Canada, and Mexico, and the prevalence of 16S-RMTase genes have increased in the last five years (2018-2022). The three species most frequently carrying 16S-RMTase genes were Acinetobacter baummannii, Klebsiella pneumoniae, and Escherichia coli. The armA gene was the most prevalent, but other 16S-RMTase genes (e.g., rmtB, rmtE, and rmtF) could be emerging backstage. More than 90% of 16S-RMTase sequences in the Americas were found in North American countries, and although the 16S-RMTase genes were less prevalent in Central and South American countries, these findings may be underestimations due to limited genomic data. Therefore, whole-genome sequence-based studies focusing on aminoglycoside resistance using a One Health approach in low- and middle-income countries should be encouraged.
摘要：
氨基糖苷类抗菌药物仍然是有价值的治疗选择，但是它们的有效性受到细菌16S核糖体RNA甲基转移酶（16S-RMTases）生产的威胁。在这项研究中,我们评估了在美洲大陆传播的革兰氏阴性菌中16S-RMTase基因的基因组流行病学.共有4877个16S-RMTase序列主要在肠杆菌和从人类分离的非发酵革兰氏阴性杆菌中被鉴定，动物,食物,和1931-2023年的环境。大多数鉴定的序列都是在美国发现的，巴西,加拿大,墨西哥,在过去五年（2018-2022年）中，16S-RMTase基因的患病率有所增加。最常携带16S-RMTase基因的三个物种是鲍曼不动杆菌，肺炎克雷伯菌,和大肠杆菌。ARMA基因是最普遍的，但其他16S-RMTase基因(例如，rmtB,rmtE,和rmtF)可能会在后台出现。美洲超过90%的16S-RMTase序列是在北美洲国家发现的，尽管16S-RMTase基因在中美洲和南美国家不太普遍，由于基因组数据有限,这些发现可能被低估.因此,应鼓励在低收入和中等收入国家采用“一个健康”方法进行以氨基糖苷类耐药为重点的全基因组序列研究.
