PDF(Pubmed)
Abstract：
The DnaA protein has long been considered to play the key role in the initiation of chromosome replication in modern bacteria. Many questions about this role, however, remain unanswered. Here, we raise these questions within a framework based on the dynamics of hyperstructures, alias large assemblies of molecules and macromolecules that perform a function. In these dynamics, hyperstructures can (1) emit and receive signals or (2) fuse and separate from one another. We ask whether the DnaA-based initiation hyperstructure acts as a logic gate receiving information from the membrane, the chromosome, and metabolism to trigger replication; we try to phrase some of these questions in terms of DNA supercoiling, strand opening, glycolytic enzymes, SeqA, ribonucleotide reductase, the macromolecular synthesis operon, post-translational modifications, and metabolic pools. Finally, we ask whether, underpinning the regulation of the cell cycle, there is a physico-chemical clock inherited from the first protocells, and whether this clock emits a single signal that triggers both chromosome replication and cell division.
摘要：
长期以来，人们一直认为DnaA蛋白在现代细菌染色体复制的启动中起关键作用。关于这个角色的很多问题，然而,仍然没有答案。这里,我们在基于超结构动力学的框架内提出这些问题，别名执行功能的分子和大分子的大型组装。在这些动态中，超结构可以（1）发射和接收信号或（2）相互融合和分离。我们询问基于DnaA的起始超结构是否充当从膜接收信息的逻辑门，染色体，和新陈代谢来触发复制；我们试图用DNA超螺旋来表达这些问题中的一些，钢绞线开口,糖酵解酶,SeqA,核糖核苷酸还原酶,大分子合成操纵子，翻译后修饰，和代谢池。最后,我们问是否，支持细胞周期的调节，第一个原始细胞遗传了一个物理化学时钟，以及这个时钟是否发出触发染色体复制和细胞分裂的单一信号。
