PDF(Pubmed)
Abstract：
This study aimed to investigate the antibiofilm and remineralising effects of peptide GAPI on artificial dentin caries. After creating artificial carious lesions, eighty dentine blocks were randomly assigned for treatment twice daily with GAPI (GAPI group) or deionised water (control group). Both groups underwent a 7-day biochemical cycle. Scanning electron microscopy (SEM) showed S. mutans with damaged structures that partially covered the dentine in the GAPI group. The dead-live ratios for the GAPI and control groups were 0.77 ± 0.13 and 0.37 ± 0.09 (p < 0.001). The log colony-forming units for the GAPI and control groups were 7.45 ± 0.32 and 8.74 ± 0.50 (p < 0.001), respectively. The lesion depths for the GAPI and control groups were 151 ± 18 µm and 214 ± 15 µm (p < 0.001), respectively. The mineral losses for the GAPI and control groups were 0.91 ± 0.07 gHAcm-3 and 1.01 ± 0.07 gHAcm-3 (p = 0.01), respectively. The hydrogen-to-amide I ratios for the GAPI and control groups were 2.92 ± 0.82 and 1.83 ± 0.73 (p = 0.014), respectively. SEM micrographs revealed fewer exposed dentine collagen fibres in the GAPI group compared to those in the control group. Furthermore, X-ray diffraction (XRD) patterns indicated that the hydroxyapatite in the GAPI group was more crystallised than that in the control group. This study demonstrated GAPI\'s antibiofilm and remineralising effects on artificial dentin caries.
摘要：
本研究旨在研究肽GAPI对人工牙本质龋的抗生物膜和再矿化作用。在制造人造龋齿病变后，80个牙本质块被随机分配用于GAPI(GAPI组)或去离子水(对照组)每日两次治疗。两组均进行7天的生化周期。扫描电子显微镜(SEM)显示具有部分覆盖GAPI组中牙本质的受损结构的变形链球菌。GAPI组和对照组的死活比分别为0.77±0.13和0.37±0.09(p<0.001)。GAPI组和对照组的对数菌落形成单位分别为7.45±0.32和8.74±0.50（p<0.001），分别。GAPI组和对照组的病变深度分别为151±18µm和214±15µm（p<0.001），分别。GAPI组和对照组的矿物质损失分别为0.91±0.07gHAcm-3和1.01±0.07gHAcm-3（p=0.01），分别。GAPI组和对照组的氢与酰胺I比率分别为2.92±0.82和1.83±0.73（p=0.014），分别。SEM显微照片显示，与对照组相比，GAPI组中暴露的牙本质胶原纤维较少。此外，X射线衍射（XRD）图表明，GAPI组中的羟基磷灰石比对照组的结晶更多。这项研究证明了GAPI对人工牙本质龋齿的抗生物膜和再矿化作用。
