Abstract:
Zea mays L. Poaceae stigma (corn silk, CS) is a byproduct of agricultural waste and is used as a traditional herb in many countries. CS is rich in chemical compounds known to benefit human health and is also a remedy for infectious diseases and has anti-proliferative effects on human cancer cell lines. In the present study, CS extract has been evaluated for its antioxidant, antibacterial, and anti-tyrosinase activities and its phytochemical composition. The higher total phenolic and flavonoid contents were found in the ethanolic extract of corn silk (CSA), at 28.27 ± 0.86 mg gallic acid equivalent/g extract and 4.71 ± 0.79 mg quercetin equivalent/g extract, respectively. Moreover, the antioxidant content of CSA was found at 5.22 ± 0.87 and 13.20 ± 0.42 mg gallic acid equivalent/g extract using DPPH and reducing power assays. Furthermore, the ethanolic extract of corn silk showed tyrosinase inhibition with an IC50 value of 12.45 µg/mL. The bacterial growth inhibition of CSA was tested using agar disc diffusion and broth dilution assays against Cutibacterium acnes and Staphylococcus epidermidis. It was found that CSA inhibited C. acnes and S. epidermidis with an inhibition zone of 11.7 ± 1.2 and 9.3 ± 0.6 mm, respectively. Moreover, the CSA showed MIC/MBC of 15.625 mg/mL against C. acnes. The following phytochemical compounds were detected in CSA: cardiac glycosides; n-hexadecanoic acid; hexadecanoic acid, ethyl ester; oleic acid; and 9,12-octadecadienoic acid, ethyl ester. After the corn silk cream product was formulated, the product demonstrated stability without phase separation. This research is beneficial for promoting effective ways to use agricultural waste while utilizing the antioxidant, anti-tyrosinase, and antibacterial activities of corn silk. Moreover, the use of technology and innovation to obtain high-value CS extract will benefit the development of commercial cosmetic products by providing safe, natural, and quality ingredients to the consumer.
摘要:
玉米禾本科柱头(玉米丝,CS)是农业废物的副产品,在许多国家被用作传统草药。CS富含已知有益于人类健康的化合物,并且也是感染性疾病的补救措施,并且对人癌细胞系具有抗增殖作用。在本研究中,CS提取物已被评估为其抗氧化剂,抗菌,和抗酪氨酸酶活性及其植物化学成分。在玉米丝(CSA)的乙醇提取物中发现了较高的总酚和类黄酮含量,在28.27±0.86毫克没食子酸当量/克提取物和4.71±0.79毫克槲皮素当量/克提取物,分别。此外,使用DPPH和还原力测定法发现CSA的抗氧化剂含量为5.22±0.87和13.20±0.42mg没食子酸当量/g提取物。此外,玉米丝的乙醇提取物显示出酪氨酸酶抑制作用,IC50值为12.45µg/mL。使用琼脂圆盘扩散和肉汤稀释测定法对CSA的细菌生长抑制作用进行了测试,以对抗痤疮杆菌和表皮葡萄球菌。发现CSA抑制痤疮梭菌和表皮葡萄球菌,抑制区分别为11.7±1.2和9.3±0.6mm,分别。此外,CSA对痤疮梭菌的MIC/MBC为15.625mg/mL。在CSA中检测到以下植物化学化合物:强心苷;正十六烷酸;十六烷酸,乙酯;油酸;和9,12-十八碳二烯酸,乙酯。玉米丝奶油产品配制后,该产品在没有相分离的情况下表现出稳定性。本研究有利于推广农业废弃物在利用抗氧化剂的同时,抗酪氨酸酶,玉米丝的抗菌活性。此外,使用技术和创新来获得高价值的CS提取物将有利于商业化妆品的开发,自然,和优质的成分给消费者。