RESULTS: In this study, we identified and characterized six insulin signalling pathway genes - InR, InR2, IRS1, PI3K1, PI3K2, and PDK - in Pardosa pseudoannulata. Real-time quantitative polymerase chain reaction results were used to analyse the relative expression levels of the six genes in different developmental instars and tissues, and in response to starvation treatment. In addition, the function of the insulin receptor substrate (IRS1) gene was investigated using RNA interference technology, which found that IRS1 significantly influenced nutrient content, developmental duration, body weight, and gonad development.
CONCLUSIONS: This study revealed the roles of six key insulin signalling pathway genes in Pardosa pseudoannulata, and in particular the importance of the IRS1 gene in regulating growth and development in the spider. The results lay the foundation for further research on the internal regulation mechanisms of growth and development in Araneae species, and also provide a reference for the artificial breeding of spiders. © 2023 Society of Chemical Industry.
结果:在这项研究中,我们鉴定并表征了六个胰岛素信号通路基因-InR,InR2、IRS1、PI3K1、PI3K2和PDK-在假环假单胞菌中。采用实时定量PCR方法分析6种基因在不同发育龄及组织中的相对表达水平,以及对饥饿治疗的反应。此外,利用RNA干扰技术研究胰岛素受体底物(IRS1)基因的功能,发现IRS1显著影响营养成分,发育持续时间,体重,和性腺发育。
结论:这项研究揭示了六个关键的胰岛素信号通路基因在假手术中的作用。特别是IRS1基因在调节蜘蛛生长发育中的重要性。研究结果为进一步研究Araneae物种生长发育的内在调控机制奠定了基础,为蜘蛛的人工繁育提供参考。本文受版权保护。保留所有权利。