PDF(Pubmed)
Abstract：
Fusarium oxysporum f. sp. cucumerinum (Foc) is a prominent pathogen that adversely affects cucumber (Cucumis sativus) production. In the pathogen\'s parasitic lifestyle, the pathogenesis and virulence evolution may be regulated by lysine acetylation, as demonstrated in many living organisms. However, its specific function in Foc remains poorly understood. In this study, the acetylome profiles of a mild virulence strain (foc-3b) and its derived virulence-enhanced strain (Ra-4) were analyzed before and post-inoculation on cucumber plants. In total, 10,664 acetylation sites were identified corresponding to 3874 proteins, and 45 conserved acetylation motifs were detected. Through comparison of the acetylomes, numerous differentially lysine-acetylated proteins were enriched in energy metabolism and protein processing processes, indicating the critical role of lysine acetylation during the transition from the saprotrophic lifestyle to the parasitic lifestyle. Comparative acetylome analyses on the two virulence-differentiated strains revealed that several differentially lysine-acetylated proteins were involved in pathways of defense response and energy metabolism. Ra-4 showed enhanced energy metabolism compared to foc-3b. This indicates that robust metabolic activity is required to achieve high virulence and facilitating adaptive evolution. Additionally, faster host responses are supported by an ample energy supply enhancing virulence. Thus, lysine acetylation plays a crucial role in the pathogenesis and virulence evolution of Foc.
摘要：
尖孢镰刀菌f.sp.黄瓜（Foc）是一种主要的病原体，对黄瓜（Cucumissativus）的生产产生不利影响。在病原体的寄生生活方式中，赖氨酸乙酰化可能调节致病和毒力进化，正如许多生物体所证明的那样。然而,它在Foc中的具体功能仍然知之甚少。在这项研究中,在黄瓜植物上接种之前和之后，分析了轻度毒力菌株（foc-3b）及其衍生的毒力增强菌株（Ra-4）的乙酰基因组谱。总的来说,鉴定出10,664个乙酰化位点，对应于3874个蛋白质，检测到45个保守的乙酰化基序。通过对乙酰基的比较，许多差异赖氨酸乙酰化蛋白在能量代谢和蛋白质加工过程中富集，表明赖氨酸乙酰化在从腐化生活方式向寄生生活方式转变过程中的关键作用。对两种毒力分化菌株的比较乙酰组分析显示，几种差异赖氨酸乙酰化蛋白参与防御反应和能量代谢途径。与foc-3b相比,Ra-4显示出增强的能量代谢。这表明需要强大的代谢活性来实现高毒力和促进适应性进化。此外,更快的宿主反应得到充足的能量供应的支持，增强毒力。因此，赖氨酸乙酰化在Foc的致病机理和毒力进化中起着至关重要的作用。
