Abstract：
The present study was designed to investigate the cerebellum histology and immunohistochemistry in porcupine (Hystrix cristata) and guinea pig (Cavia porcellus). Two adult porcupines and two adult guinea pigs were used. For general histology, crystal violet and Luxol fast blue stains were applied. For immunohistochemistry, myelin-associated glycoprotein (MAG), neurofilament 200 (NF200), calbindin D-28K, and glial fibrillary-associated protein (GFAP) were investigated. The cerebellar cortex in both species was composed of three cellular layers: molecular, granular, and Purkinje cell (PC) layers. Purkinje cells in the porcupine showed a purple-colored and dark blue-colored cytoplasm in reaction to the crystal violet and Luxol fast blue staining, respectively. In the guinea pig, PC has a uniform reaction to the Luxol fast blue with dark-blue-colored cytoplasm. However, in response to the crystal violet, some PC with dark-purple cytoplasm showed stronger reaction than other PC which showed light-purple cytoplasm. The PC layer in some folia of the porcupine cerebellum was composed of 2-3 layers. The expression rates of calbindin D-28K, MAG, GFAP, and NF200 in the porcupine cerebellum were determined to be 19%, 42.5%, 62%, and 30%, respectively. These values were determined to be 27%, 34%, 43.5%, and 31.5%, respectively, in the guinea pig cerebellum.
摘要：
本研究旨在研究豪猪（Hystrixcristata）和豚鼠（Capiaporcellus）的小脑组织学和免疫组织化学。使用两只成年豪猪和两只成年豚鼠。对于一般组织学，应用结晶紫和Luxol快速蓝色染色剂。对于免疫组织化学，髓鞘相关糖蛋白（MAG），神经丝200(NF200),CalbindinD-28K,和胶质纤维相关蛋白（GFAP）进行了研究。这两个物种的小脑皮质由三层细胞层组成：分子，颗粒状,和浦肯野细胞（PC）层。豪猪中的Purkinje细胞对结晶紫和Luxol快速蓝染色反应显示出紫色和深蓝色的细胞质，分别。在豚鼠身上，PC对具有深蓝色细胞质的Luxol快速蓝具有均匀的反应。然而,作为对结晶紫的回应，一些深紫色细胞质的PC比其他呈浅紫色细胞质的PC表现出更强的反应。豪猪小脑某些叶中的PC层由2-3层组成。钙结合蛋白D-28K的表达率,MAG,GFAP,豪猪小脑中的NF200被确定为19%，42.5%,62%,30%,分别。这些值被确定为27%，34%,43.5%,和31.5%，分别,在豚鼠小脑.
