Abstract:
Serum protein electrophoresis has been demonstrated to have utility in diagnostic workup, wellness exams, and prognosis. Agarose gel electrophoresis (AGE) has previously been described for use with serum from Asian elephants (Elephas maximus). As the newer method of capillary zone electrophoresis (CZE) is becoming more commonplace in veterinary diagnostic laboratories, serum samples from Asian elephants were examined using this method. CZE allowed for a reproducible definition of two beta fractions and, overall, showed a low coefficient of variation for fraction quantitation. Preliminary reference intervals were generated using samples primarily from an older population of 22 female elephants. Albumin levels determined by CZE were also compared with those determined by the bromocresol green method on a chemistry analyzer. It was found that the latter method overestimated the level of albumin with a mean positive bias of 11.6% or 0.38 g/dL, thus method-specific reference intervals should be used. Significant negative correlations were observed between A/G ratio determined by CZE and serum amyloid A levels (r = -0.47, p < 0.0001) and haptoglobin (r = -0.52, p < 0.0001); both APP were significantly correlated with the alpha 2 globulin fraction (p < 0.0001). CZE reflects an overall picture of changes in acute phase proteins and immunoglobulins and accurate quantitation of albumin and thus should be considered as an adjunct tool to the use of other measures of the acute phase response in patient monitoring.
摘要:
血清蛋白电泳已被证明在诊断检查中具有实用性,健康考试,和预后。先前已描述了琼脂糖凝胶电泳(AGE)与来自亚洲象(Elephasmaximus)的血清一起使用。随着毛细管区带电泳(CZE)的新方法在兽医诊断实验室中变得越来越普遍,使用这种方法检查了亚洲象的血清样本。CZE允许两个β级分的可重复定义,总的来说,显示分数定量的变异系数低。初步参考间隔是使用主要来自22头雌性大象的老年人群的样本生成的。还在化学分析仪上比较了通过CZE测定的白蛋白水平与通过溴甲酚绿方法测定的白蛋白水平。发现后一种方法高估了白蛋白的水平,平均正偏差为11.6%或0.38g/dL,因此,应使用特定方法的参考间隔。CZE测定的A/G比与血清淀粉样蛋白A水平(r=-0.47,p<0.0001)和结合珠蛋白(r=-0.52,p<0.0001)之间存在显着负相关;两种APP均与α2球蛋白分数显着相关(p<0.0001)。CZE反映了急性期蛋白和免疫球蛋白变化的总体情况以及白蛋白的准确定量,因此应被视为在患者监测中使用其他急性期反应措施的辅助工具。