PDF(Pubmed)
Abstract：
OBJECTIVE: To compare the use of toluidine blue 1% eye drops with anterior segment optical coherence tomography (OCT) for the determination of tumour margins in patients with ocular surface squamous neoplasia (OSSN).
METHODS: The study was conducted from July 2020 to June 2021 at the Ocular Oncology department at the Federal University of São Paulo, Brazil. Slit-lamp photographs after toluidine blue staining and OCT of the anterior segment were taken on the same day from patients with OSSN. Photographs and OCT images were analyzed quantitatively using the software ImageJ and IMAGEnet®, respectively. The agreement between techniques was evaluated qualitatively through the Bland-Altman graph and quantitatively through intraclass correlation (ICC).
RESULTS: A total of 21 participants (71.43% males) with a clinical diagnosis of OSSN were included in the study. The average + SD diameter along the chosen axes was 4.43 ± 2.08 mm with OCT of 4.37 ± 2.03 mm with toluidine blue, a difference not statistically significant (p = 0.2891). The Bland-Altman analysis indicated a good qualitative agreement between the methods, with all cases inserted within the limits of agreement from -0.3217 to 0.4268. The ICC quantitative analysis showed an almost perfect agreement of 99.57% (95%CI: 98.96-99.83%; p < 0.001).
CONCLUSIONS: Our findings showed that OCT and toluidine eye drops are equivalent in determining margins for tumour measurements, which is particularly relevant in low-income settings where anterior segment OCT is not available. The use of toluidine blue 1% could be an useful alternative to quantify the size of the tumour, help to monitor tumour growth, and outline margins for surgical planning.
METHODS: The study was conducted from July 2020 to June 2021 at the Ocular Oncology department at the Federal University of São Paulo, Brazil. Slit-lamp photographs after toluidine blue staining and OCT of the anterior segment were taken on the same day from patients with OSSN. Photographs and OCT images were analyzed quantitatively using the software ImageJ and IMAGEnet®, respectively. The agreement between techniques was evaluated qualitatively through the Bland-Altman graph and quantitatively through intraclass correlation (ICC).
RESULTS: A total of 21 participants (71.43% males) with a clinical diagnosis of OSSN were included in the study. The average + SD diameter along the chosen axes was 4.43 ± 2.08 mm with OCT of 4.37 ± 2.03 mm with toluidine blue, a difference not statistically significant (p = 0.2891). The Bland-Altman analysis indicated a good qualitative agreement between the methods, with all cases inserted within the limits of agreement from -0.3217 to 0.4268. The ICC quantitative analysis showed an almost perfect agreement of 99.57% (95%CI: 98.96-99.83%; p < 0.001).
CONCLUSIONS: Our findings showed that OCT and toluidine eye drops are equivalent in determining margins for tumour measurements, which is particularly relevant in low-income settings where anterior segment OCT is not available. The use of toluidine blue 1% could be an useful alternative to quantify the size of the tumour, help to monitor tumour growth, and outline margins for surgical planning.
摘要：
目的：比较1%甲苯胺蓝滴眼液与眼前节光学相干断层扫描（OCT）测定眼表鳞状细胞瘤（OSSN）患者的肿瘤边缘。
方法：该研究于2020年7月至2021年6月在圣保罗联邦大学眼部肿瘤学系进行，巴西。同一天，从OSSN患者中拍摄甲苯胺蓝染色和眼前节OCT后的裂隙灯照片。使用ImageJ和IMAGEnet®软件对照片和OCT图像进行定量分析。分别。技术之间的一致性通过Bland-Altman图进行定性评估，并通过组内相关性（ICC）进行定量评估。
结果：共有21名临床诊断为OSSN的参与者（男性占71.43%）被纳入研究。选择轴的平均+SD直径为4.43±2.08mm,使用甲苯胺蓝的OCT为4.37±2.03mm,差异无统计学意义(p=0.2891)。Bland-Altman分析表明两种方法之间有很好的定性一致性，在-0.3217至0.4268的协议范围内插入所有案件。ICC定量分析显示99.57％的几乎完美一致性（95CI：98.96-99.83％；p<0.001）。
结论：我们的研究结果表明，OCT和甲苯胺滴眼液在确定肿瘤测量的边缘方面是等效的，这在前节OCT不可用的低收入环境中尤其重要。使用甲苯胺蓝1％可能是量化肿瘤大小的有用替代方法，帮助监测肿瘤生长，并勾勒出手术计划的边缘。
方法：该研究于2020年7月至2021年6月在圣保罗联邦大学眼部肿瘤学系进行，巴西。同一天，从OSSN患者中拍摄甲苯胺蓝染色和眼前节OCT后的裂隙灯照片。使用ImageJ和IMAGEnet®软件对照片和OCT图像进行定量分析。分别。技术之间的一致性通过Bland-Altman图进行定性评估，并通过组内相关性（ICC）进行定量评估。
结果：共有21名临床诊断为OSSN的参与者（男性占71.43%）被纳入研究。选择轴的平均+SD直径为4.43±2.08mm,使用甲苯胺蓝的OCT为4.37±2.03mm,差异无统计学意义(p=0.2891)。Bland-Altman分析表明两种方法之间有很好的定性一致性，在-0.3217至0.4268的协议范围内插入所有案件。ICC定量分析显示99.57％的几乎完美一致性（95CI：98.96-99.83％；p<0.001）。
结论：我们的研究结果表明，OCT和甲苯胺滴眼液在确定肿瘤测量的边缘方面是等效的，这在前节OCT不可用的低收入环境中尤其重要。使用甲苯胺蓝1％可能是量化肿瘤大小的有用替代方法，帮助监测肿瘤生长，并勾勒出手术计划的边缘。
