PDF(Pubmed)
Abstract：
Food allergies are a growing public health problem with recent estimates of 10% of the US population affected by this immunologic disease. The quality of life is greatly impaired in food allergic individuals and their caregivers due to constant vigilance and fear of accidental exposure. Shellfish allergies are of particular concern because their prevalence has increased over the past 15 years, now affecting an estimated 3% of the adult population and 1.3% of children in the USA. Additionally, they are rarely outgrown, can result in fatal reactions, and there are no FDA-approved therapies for shellfish allergies. Reactions to one type of shellfish, crustaceans (shrimp, lobster, and crab), can be especially severe. The major crustacean allergens are highly conserved across species, resulting in high cross-reactivity of IgE between shrimp, lobster, and crab in allergic individuals. To develop novel therapies for shellfish allergies, preclinical mouse models are required. In this chapter, we present detailed methodology to induce shrimp allergy in CC027 mice. Once sensitized, mice produce shrimp-specific IgE, that is cross-reactive with lobster and crab, and experience anaphylaxis upon shrimp challenge. This model can be used to further investigate mechanisms of sensitization and preclinical testing of therapies.
摘要：
食物过敏是一个日益严重的公共卫生问题，最近估计有10％的美国人口受到这种免疫疾病的影响。由于持续的警惕和对意外暴露的恐惧，食物过敏个体及其护理人员的生活质量受到极大损害。贝类过敏尤其令人担忧，因为它们的患病率在过去15年中有所增加，现在影响了美国大约3%的成年人和1.3%的儿童。此外,他们很少长大，会导致致命的反应，也没有FDA批准的贝类过敏疗法。对一种贝类的反应,甲壳类动物（虾，龙虾,和螃蟹），可能特别严重。主要的甲壳类过敏原在物种之间高度保守，导致虾之间IgE的高交叉反应性，龙虾,和过敏个体的螃蟹。开发贝类过敏的新疗法，临床前小鼠模型是必需的。在这一章中,我们提出了在CC027小鼠中诱导虾过敏的详细方法。一旦敏感，小鼠产生虾特异性IgE，与龙虾和螃蟹交叉反应，并在虾挑战时经历过敏反应。该模型可用于进一步研究致敏机制和治疗的临床前测试。
