Abstract：
Several drought and salt tolerant phenotypes have been reported when overexpressing (OE) phospholipase C (PLC) genes across plant species. In contrast, a negative role for Arabidopsis PLC4 in salinity stress was recently proposed, showing that roots of PLC4-OE seedlings were more sensitive to NaCl while plc4 knock-out (KO) mutants were more tolerant. To investigate this apparent contradiction, and to analyse the phospholipid signalling responses associated with salinity stress, we performed root growth- and phospholipid analyses on plc4-KO and PLC4-OE seedlings subjected to salinity (NaCl) or osmotic (sorbitol) stress and compared these with wild type (WT). Only very minor differences between PLC4 mutants and WT were observed, which even disappeared after normalization of the data, while in soil, PLC4-OE plants were clearly more drought tolerant than WT plants, as was found earlier when overexpressing Arabidopsis PLC2, -3, -5, -7 or -9. We conclude that PLC4 plays no opposite role in salt-or osmotic stress and rather behaves like the other Arabidopsis PLCs.
摘要：
在跨植物物种过表达（OE）磷脂酶C（PLC）基因时，已经报道了几种耐旱和耐盐表型。相比之下,最近提出了拟南芥PLC4在盐度胁迫中的负面作用，表明PLC4-OE幼苗的根对NaCl更敏感，而plc4敲除（KO）突变体的耐受性更高。为了调查这个明显的矛盾，并分析与盐度胁迫相关的磷脂信号反应，我们对受到盐度（NaCl）或渗透（山梨糖醇）胁迫的plc4-KO和PLC4-OE幼苗进行了根生长和磷脂分析，并将其与野生型（WT）进行了比较。仅观察到PLC4突变体和WT之间的非常微小的差异,它甚至在数据标准化后消失了，在土壤中，PLC4-OE植物明显比WT植物更耐旱性。如先前在过表达拟南芥PLC2、-3、-5、-7或-9时发现的。我们得出的结论是，PLC4在盐或渗透胁迫中没有相反的作用，并且表现得像其他拟南芥PLC一样。
