Abstract:
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized diagnostics in culture-based microbiology. Commonly used MALDI-TOF MS systems in clinical microbiology laboratories are MALDI Biotyper (Bruker Daltonics) and Vitek MS (bioMérieux), but recently the new EXS2600 (Zybio) has been launched. This study aimed to evaluate the performance of the three devices by comparing the results to 16S rRNA gene sequencing. A set of 356 previously collected difficult-to-identify bacteria was tested in parallel with the three systems. Only the direct smear method and simple formic acid extraction were applied. Valid results were achieved for 98.6%, 94.4%, and 93.3% of all isolates by MALDI Biotyper, EXS2600, and Vitek MS, respectively. Of all valid results, agreement with sequencing data was achieved in 98.9%, 98.5%, and 99.7% by MALDI Biotyper, EXS2600, and Vitek MS, respectively. Considering only the isolates with valid measurements at the single-species level, misidentification rates were 0%, 2.6%, and 1.1% for MALDI Biotyper, EXS2600, and Vitek MS, respectively. Apart from minor performance differences, our data demonstrate that the three systems provide comparable results and are suitable for use in medical diagnostic laboratories.
摘要:
基质辅助激光解吸/电离飞行时间质谱(MALDI-TOFMS)彻底改变了基于培养的微生物学诊断。临床微生物学实验室中常用的MALDI-TOFMS系统是MALDIBiotyper(BrukerDaltonics)和VitekMS(bioMérieux),但最近新的EXS2600(Zybio)已经推出。本研究旨在通过将结果与16SrRNA基因测序进行比较来评估三种设备的性能。一组356个先前收集的难以识别的细菌与三个系统并行测试。仅采用直接涂片法和简单的甲酸提取法。98.6%取得了有效结果,94.4%,和93.3%的所有分离株由MALDIBiotyper,EXS2600和VitekMS,分别。在所有有效的结果中,与测序数据的一致性达到98.9%,98.5%,MALDIBiotyper的99.7%,EXS2600和VitekMS,分别。仅考虑在单物种水平上具有有效测量值的分离株,错误识别率为0%,2.6%,MALDIBiotyper为1.1%,EXS2600和VitekMS,分别。除了轻微的性能差异,我们的数据表明,这三种系统提供了相当的结果,适用于医学诊断实验室.