PDF(Pubmed)
Abstract：
Sebaceous glands (SGs) release oils that protect our skin, but how these glands respond to injury has not been previously examined. Here, we report that SGs are largely self-renewed by dedicated stem cell pools during homeostasis. Using targeted single-cell RNA sequencing, we uncovered both direct and indirect paths by which resident SG progenitors ordinarily differentiate into sebocytes, including transit through a Krt5+PPARγ+ transitional basal cell state. Upon skin injury, however, SG progenitors depart their niche, reepithelialize the wound, and are replaced by hair-follicle-derived stem cells. Furthermore, following targeted genetic ablation of >99% of SGs from dorsal skin, these glands unexpectedly regenerate within weeks. This regenerative process is mediated by alternative stem cells originating from the hair follicle bulge, is dependent upon FGFR2 signaling, and can be accelerated by inducing hair growth. Altogether, our studies demonstrate that stem cell plasticity promotes SG durability following injury.
摘要：
皮脂腺（SGs）释放油，保护我们的皮肤，但是这些腺体对损伤的反应尚未得到研究。这里,我们报告说,在稳态期间,SGs主要通过专用干细胞池进行自我更新.使用靶向单细胞RNA测序，我们发现了常驻SG祖细胞通常分化为皮脂腺细胞的直接和间接途径，包括通过Krt5+PPARγ+过渡基底细胞状态的转运。皮肤受伤时,然而,SG祖细胞离开了他们的生态位，伤口重新上皮化，并被毛囊来源的干细胞所取代。此外，在对背侧皮肤>99%的SGs进行靶向遗传消融后，这些腺体在几周内意外再生。这种再生过程是由源自毛囊凸起的替代干细胞介导的，依赖于FGFR2信号，并且可以通过诱导头发生长来加速。总之,我们的研究表明,干细胞可塑性可促进损伤后SG的耐久性.
