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Abstract：
BACKGROUND: The role of human parvovirus B19 (B19V) infection in malignant and benign lesions such as head and neck squamous cell carcinomas (HNSCCs) and oral mucocele lesions has not been established. Herein, we examined, for the first time, the presence of B19V in HNSCCs from Iranian subjects.
METHODS: One hundred and eight HNSCC specimens were analyzed for the presence of B19V using nested polymerase chain reaction (nPCR) and TaqMan quantitative PCR assays. Immunohistochemistry procedures were performed to evaluate the expression of B19V VP1/VP2 proteins, p16INK4a, and NF-κB in tumor tissues and their adjacent non-tumor tissues. In addition, 40 oral mucocele, 30 oral buccal mucosa swabs, and 30 nasopharyngeal swabs obtained from healthy adults were analyzed as controls.
RESULTS: B19V DNA was detected in 36.1% of HNSCCs. Further, 23.3% of HNSCC specimens showed immunoreactivity against B19V VP1/VP2 proteins. There was a significant difference in the frequency of B19V DNA-positive cases between the patient and control groups (p < 0.0001). Moreover, comparing tumoral tissues and their adjacent non-tumor tissues in terms of immunoreactivity against B19V structural proteins, a significant association was found between tumor tissues and B19V infection (p < 0.0001). Finally, investigating the simultaneous presence of B19V and high-risk human papillomaviruses (HPV) DNA, we found a significant association between these two viral infections in HNSCCs (p = 0.031).
CONCLUSIONS: To sum up, B19V was frequently present in HNSCC tissues of Iranian patients but mostly absent in the adjacent non-tumor tissues as well as oral mucocele lesions, buccal, and nasopharyngeal swabs of healthy subjects. HPV possibly contributes to B19V persistence in HNSCC tissues. Additional research is required to investigate potential etiological or cofactor roles of B19V in the development of HNSCCs.
METHODS: One hundred and eight HNSCC specimens were analyzed for the presence of B19V using nested polymerase chain reaction (nPCR) and TaqMan quantitative PCR assays. Immunohistochemistry procedures were performed to evaluate the expression of B19V VP1/VP2 proteins, p16INK4a, and NF-κB in tumor tissues and their adjacent non-tumor tissues. In addition, 40 oral mucocele, 30 oral buccal mucosa swabs, and 30 nasopharyngeal swabs obtained from healthy adults were analyzed as controls.
RESULTS: B19V DNA was detected in 36.1% of HNSCCs. Further, 23.3% of HNSCC specimens showed immunoreactivity against B19V VP1/VP2 proteins. There was a significant difference in the frequency of B19V DNA-positive cases between the patient and control groups (p < 0.0001). Moreover, comparing tumoral tissues and their adjacent non-tumor tissues in terms of immunoreactivity against B19V structural proteins, a significant association was found between tumor tissues and B19V infection (p < 0.0001). Finally, investigating the simultaneous presence of B19V and high-risk human papillomaviruses (HPV) DNA, we found a significant association between these two viral infections in HNSCCs (p = 0.031).
CONCLUSIONS: To sum up, B19V was frequently present in HNSCC tissues of Iranian patients but mostly absent in the adjacent non-tumor tissues as well as oral mucocele lesions, buccal, and nasopharyngeal swabs of healthy subjects. HPV possibly contributes to B19V persistence in HNSCC tissues. Additional research is required to investigate potential etiological or cofactor roles of B19V in the development of HNSCCs.
摘要：
背景：人类细小病毒B19（B19V）感染在恶性和良性病变如头颈部鳞状细胞癌（HNSCC）和口腔粘膜膨出病变中的作用尚未确定。在这里，我们检查了,第一次,来自伊朗受试者的HNSCC中B19V的存在。
方法：使用巢式聚合酶链反应（nPCR）和TaqMan定量PCR分析了108个HNSCC样本中B19V的存在。进行免疫组织化学程序以评估B19VVP1/VP2蛋白的表达。p16INK4a,和NF-κB在肿瘤组织及其邻近的非肿瘤组织中的表达。此外,40口黏液囊肿,30个口腔颊粘膜拭子，30例健康成人鼻咽拭子作为对照进行分析.
结果：在36.1%的HNSCC中检测到B19VDNA。Further,23.3％的HNSCC样本显示针对B19VVP1/VP2蛋白的免疫反应性。患者组和对照组之间B19VDNA阳性病例的频率存在显著差异(p<0.0001)。此外，比较肿瘤组织及其邻近的非肿瘤组织对B19V结构蛋白的免疫反应性，发现肿瘤组织与B19V感染之间存在显著关联(p<0.0001).最后,调查同时存在的B19V和高危人乳头瘤病毒（HPV）DNA，我们发现HNSCCs中这两种病毒感染之间存在显著关联(p=0.031).
结论：总而言之，B19V经常出现在伊朗患者的HNSCC组织中，但在邻近的非肿瘤组织以及口腔粘膜膨出病变中大多不存在。颊,和健康受试者的鼻咽拭子。HPV可能有助于HNSCC组织中的B19V持续。需要额外的研究来调查B19V在HNSCC发展中的潜在病因或辅因子作用。
方法：使用巢式聚合酶链反应（nPCR）和TaqMan定量PCR分析了108个HNSCC样本中B19V的存在。进行免疫组织化学程序以评估B19VVP1/VP2蛋白的表达。p16INK4a,和NF-κB在肿瘤组织及其邻近的非肿瘤组织中的表达。此外,40口黏液囊肿,30个口腔颊粘膜拭子，30例健康成人鼻咽拭子作为对照进行分析.
结果：在36.1%的HNSCC中检测到B19VDNA。Further,23.3％的HNSCC样本显示针对B19VVP1/VP2蛋白的免疫反应性。患者组和对照组之间B19VDNA阳性病例的频率存在显著差异(p<0.0001)。此外，比较肿瘤组织及其邻近的非肿瘤组织对B19V结构蛋白的免疫反应性，发现肿瘤组织与B19V感染之间存在显著关联(p<0.0001).最后,调查同时存在的B19V和高危人乳头瘤病毒（HPV）DNA，我们发现HNSCCs中这两种病毒感染之间存在显著关联(p=0.031).
结论：总而言之，B19V经常出现在伊朗患者的HNSCC组织中，但在邻近的非肿瘤组织以及口腔粘膜膨出病变中大多不存在。颊,和健康受试者的鼻咽拭子。HPV可能有助于HNSCC组织中的B19V持续。需要额外的研究来调查B19V在HNSCC发展中的潜在病因或辅因子作用。
