PDF(Pubmed)
Abstract：
BACKGROUND: Mycoplasma hominis is a facultative anaerobic bacterium commonly present in the urogenital tract. In recent years, M. hominis has increasingly been associated with extra-urogenital tract infections, particularly in immunosuppressed patients. Detecting M. hominis in a diagnostic laboratory can be challenging due to its slow growth rate, absence of a cell wall, and the requirements of specialized media and conditions for optimal growth. Consequently, it is necessary to establish guidelines for the detection of this microorganism and to request the appropriate microbiological work-up of immunosuppressed patients.
METHODS: We hereby present two cases of solid organ transplant patients who developed M. hominis infection. Microscopic examination of the bronchial lavage and pleural fluid showed no microorganisms. However, upon inoculating the specimens onto routine microbiology media, the organism was successfully identified and confirmation was performed using 16S rDNA sequencing. Both patients received appropriate treatment resulting in the resolution of M. hominis infection.
CONCLUSIONS: The prompt detection of M. hominis in a clinical specimen can have a significant impact on patient care by allowing for early intervention and ultimately resulting in more favorable clinical outcomes, especially in transplant patients.
METHODS: We hereby present two cases of solid organ transplant patients who developed M. hominis infection. Microscopic examination of the bronchial lavage and pleural fluid showed no microorganisms. However, upon inoculating the specimens onto routine microbiology media, the organism was successfully identified and confirmation was performed using 16S rDNA sequencing. Both patients received appropriate treatment resulting in the resolution of M. hominis infection.
CONCLUSIONS: The prompt detection of M. hominis in a clinical specimen can have a significant impact on patient care by allowing for early intervention and ultimately resulting in more favorable clinical outcomes, especially in transplant patients.
摘要：
背景：人型支原体是一种兼性厌氧菌，通常存在于泌尿生殖道。近年来,人分枝杆菌越来越多地与泌尿生殖道外感染有关，特别是在免疫抑制患者中。在诊断实验室中检测人源分枝杆菌可能由于其缓慢的生长速度而具有挑战性，没有细胞壁,以及专业培养基的要求和最佳生长条件。因此,有必要建立这种微生物的检测指南，并要求对免疫抑制患者进行适当的微生物检查。
方法：我们在此介绍两例发生人型支原体感染的实体器官移植患者。支气管灌洗和胸膜液的显微镜检查未显示微生物。然而,在将样本接种到常规微生物学培养基上时，成功鉴定了该生物体,并使用16SrDNA测序进行了确认.两名患者均接受了适当的治疗，从而解决了人支原体感染。
结论：在临床标本中迅速检测人源M.hominis可以通过早期干预并最终导致更有利的临床结果，从而对患者护理产生重大影响。尤其是移植患者。
方法：我们在此介绍两例发生人型支原体感染的实体器官移植患者。支气管灌洗和胸膜液的显微镜检查未显示微生物。然而,在将样本接种到常规微生物学培养基上时，成功鉴定了该生物体,并使用16SrDNA测序进行了确认.两名患者均接受了适当的治疗，从而解决了人支原体感染。
结论：在临床标本中迅速检测人源M.hominis可以通过早期干预并最终导致更有利的临床结果，从而对患者护理产生重大影响。尤其是移植患者。
