Abstract：
Objective: We aim to investigate the species composition of ticks and the pathogen characteristics they carry in the Argun port area of the China-Russia border. Materials and Methods: Ticks were collected in surrounding grassland, mixed forest land, and other different habitats around the Argun port area at the Sino-Russian Border of Inner Mongolia in China in April 2019. The presence of 16 potential pathogens, including Yersinia Pestis, Francisella tularensis, Coxiella burnetii (Cb), Anaplasma sp. (Ap), spotted fever group rickettsiae (SFG Rk), Borrelia sp. (Bl), Leptospira, Bartonella spp., Babesia, Crimean-Congo hemorrhagic fever virus, tick-borne encephalitis virus, Bhanja virus, West Nile Virus, severe fever with thrombocytopenia syndrome bunyavirus, Hantaan virus, and bocavirus (boca) was analyzed by polymerase chain reaction. The DNA and amino acid sequences of tick-borne pathogens were compared for homology, and the phylogenetic trees were constructed by using Mega and Lasergene software. Results: A total of 210 ticks were collected and they belonged to three species: Dermacentor nuttalli, Ixodes persulcatus, and Haemaphysalis verticalis. Among them, 165 (78.57%) ticks tested positive for 5 pathogens, namely Ap, SFG Rk, Cb, Bl, and boca. Fifteen (7.14%) ticks were detected coinfection with two pathogens, and none were coinfected with three or more pathogens. Conclusion: This study shows the prevalence of at least five tick-borne pathogens in Argun, and there is a risk of coinfection by two pathogens in one tick. This study reveals the great importance of controlling tick-borne diseases in this region.
摘要：
目的:研究中俄边境阿尔贡口岸蜱的种类组成和病原特征。材料和方法：在周围的草地上收集蜱，混合林地,以及2019年4月中国内蒙古中俄边境阿尔贡口岸区周边其他不同栖息地。16种潜在病原体的存在，包括鼠疫耶尔森氏菌,图拉西斯,伯氏柯西拉（Cb），无菌血症。(Ap),斑点热组立克次体（SFGRk），疏螺旋体sp.(Bl),钩端螺旋体,巴尔通菌属。,巴贝西亚,克里米亚-刚果出血热病毒,蜱传脑炎病毒，Bhanja病毒,西尼罗河病毒,严重发热伴血小板减少综合征布尼亚病毒，汉坦病毒,和博卡病毒（bocavirus，boca）通过聚合酶链反应进行分析。对蜱传病原体的DNA和氨基酸序列进行同源性比较,并利用Mega和Lasergene软件构建了系统发育树。结果：共收集到210只蜱，它们属于三个物种：NuttalliDermacentor，伊科雷克斯，和verticalis。其中,165只(78.57%)蜱对5种病原体检测呈阳性，即Ap,SFGRk,Cb,Bl,还有Boca.检测到15只(7.14%)蜱与两种病原体共感染，没有人同时感染三种或三种以上病原体。结论：这项研究表明，在Argun中至少有五种蜱传病原体的患病率，一个蜱有两种病原体共同感染的风险。这项研究揭示了在该地区控制蜱传疾病的重要性。
