Abstract：
BACKGROUND: Behçet\'s disease (BD) has a growing prevalence in Silk Road countries. The aim of our cross-sectional study was to explore the clinical and molecular predictors of quality of life in BD patients.
METHODS: One hundred and fifty consecutive Iranian BD patients with an age range between 20-50 years were included. The Leeds Behçet\'s disease quality of life (BDQoL) in Persian form was fulfilled to evaluate the quality of life. Anthropometric measurements were carried out using the calibrated scales. Iranian Behcet\'s Disease Dynamic Activity Measure (IBDDAM), Behcet\'s disease current activity form (BDCAF), and Total Inflammatory Activity Index (TIAI) were used to assess BD activity. mRNA expression of toll-like receptors 2 and 4 (TLR2 and TLR4) and tumor-necrosis-factor-alpha (TNF-α) levels in serum were measured by real-time polymerase chain reaction (PCR) and ELISA, respectively. Multiple linear backward regression at P = 0.1 was used to study the potential predictors of quality of life.
RESULTS: TLR2 and BDCAF were shown to be the most important predictors of quality of life in BD patients by 22%. There were positive associations between them (β = 0.326, p = 0.013 for BDCAF; β = 0.366, p = 0.006 for TLR2) and BDQoL value.
CONCLUSIONS: Higher TLR2 expression as a key protein in recognizing pathogens by innate immunity and BDCAF value as a comprehensive BD assessing scale contribute to poor quality of life among BD patients. Emphasizing therapeutically, approaches associated with lower TLR2 expression and BDCAF value can be considered in future studies.
METHODS: One hundred and fifty consecutive Iranian BD patients with an age range between 20-50 years were included. The Leeds Behçet\'s disease quality of life (BDQoL) in Persian form was fulfilled to evaluate the quality of life. Anthropometric measurements were carried out using the calibrated scales. Iranian Behcet\'s Disease Dynamic Activity Measure (IBDDAM), Behcet\'s disease current activity form (BDCAF), and Total Inflammatory Activity Index (TIAI) were used to assess BD activity. mRNA expression of toll-like receptors 2 and 4 (TLR2 and TLR4) and tumor-necrosis-factor-alpha (TNF-α) levels in serum were measured by real-time polymerase chain reaction (PCR) and ELISA, respectively. Multiple linear backward regression at P = 0.1 was used to study the potential predictors of quality of life.
RESULTS: TLR2 and BDCAF were shown to be the most important predictors of quality of life in BD patients by 22%. There were positive associations between them (β = 0.326, p = 0.013 for BDCAF; β = 0.366, p = 0.006 for TLR2) and BDQoL value.
CONCLUSIONS: Higher TLR2 expression as a key protein in recognizing pathogens by innate immunity and BDCAF value as a comprehensive BD assessing scale contribute to poor quality of life among BD patients. Emphasizing therapeutically, approaches associated with lower TLR2 expression and BDCAF value can be considered in future studies.
摘要：
背景：Behçet病（BD）在丝绸之路国家的患病率越来越高。我们横断面研究的目的是探讨BD患者生活质量的临床和分子预测因素。
方法：纳入150例年龄在20-50岁之间的伊朗BD患者。实现了波斯形式的利兹·贝赫塞特疾病生活质量（BDQoL），以评估生活质量。使用校准的标尺进行人体测量。伊朗Behcet疾病动态活动测量(IBDDAM)，Behcet疾病当前活动形式（BDCAF），和总炎症活动指数(TIAI)用于评估BD活性。通过实时聚合酶链反应（PCR）和ELISA检测血清中Toll样受体2和4（TLR2和TLR4）的mRNA表达和肿瘤坏死因子-α（TNF-α）水平。分别。P=0.1的多元线性后向回归用于研究生活质量的潜在预测因素。
结果：显示TLR2和BDCAF是BD患者生活质量的最重要预测因子，占22％。它们之间存在正相关（BDCAF的β=0.326，p=0.013；TLR2的β=0.366，p=0.006）和BDQoL值之间存在正相关。
结论：TLR2作为先天免疫识别病原体的关键蛋白表达较高，BDCAF值作为综合BD评估量表导致BD患者生活质量较差。在未来的研究中可以考虑强调与较低TLR2表达和BDCAF值相关的治疗方法。
方法：纳入150例年龄在20-50岁之间的伊朗BD患者。实现了波斯形式的利兹·贝赫塞特疾病生活质量（BDQoL），以评估生活质量。使用校准的标尺进行人体测量。伊朗Behcet疾病动态活动测量(IBDDAM)，Behcet疾病当前活动形式（BDCAF），和总炎症活动指数(TIAI)用于评估BD活性。通过实时聚合酶链反应（PCR）和ELISA检测血清中Toll样受体2和4（TLR2和TLR4）的mRNA表达和肿瘤坏死因子-α（TNF-α）水平。分别。P=0.1的多元线性后向回归用于研究生活质量的潜在预测因素。
结果：显示TLR2和BDCAF是BD患者生活质量的最重要预测因子，占22％。它们之间存在正相关（BDCAF的β=0.326，p=0.013；TLR2的β=0.366，p=0.006）和BDQoL值之间存在正相关。
结论：TLR2作为先天免疫识别病原体的关键蛋白表达较高，BDCAF值作为综合BD评估量表导致BD患者生活质量较差。在未来的研究中可以考虑强调与较低TLR2表达和BDCAF值相关的治疗方法。
