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Abstract：
A response surface methodology (RSM) with a central composite design (CCD) was developed to predict and apply the best ultrasound-assisted extraction (UAE) conditions, including the extraction time, the composition of aqueous-ethanolic extractants, and the solvent-to-plant-material ratio, for obtaining the highest yields of different types of polyphenolic components from the dried flower buds of Magnolia × soulangeana Soul.-Bod. var. \'Lennei\' (MSL). The novel approach in the RSM procedure resulted from the simultaneous optimisation of UAE conditions to obtain extracts with the highest antioxidant and antiradical potential (examined as dependent variables), using appropriate spectrophotometric assays, with Folin-Ciocâlteu and 2,2-diphenyl-1-picrylhydrazyl reagents, respectively. The use of 66.8% (V/V) ethanol as the extraction solvent during the 55.2 min extraction protocol and the ratio of extractant volume to herbal substance of 46.8 mL/g gave the highest total yield of bioactive antioxidant phenolics in the extract obtained. For this herbal preparation, a qualitative and quantitative analysis was performed using combined chromatographic (LC), spectroscopic (PDA), and tandem mass spectrometric (ESI-QToF-MS/MS) techniques. A detailed phytochemical profiling, conducted for the first time, documented substantial amounts of various polyphenolic antioxidants, especially phenylethanoids and flavonoids, in the MSL flower buds. Their average total content exceeded 30.3 and 36.5 mg/g dry weight, respectively.
摘要：
开发了具有中央复合设计（CCD）的响应面方法（RSM），以预测和应用最佳的超声辅助提取（UAE）条件，包括提取时间，含水乙醇萃取剂的组成，和溶剂与植物材料的比例，从木兰×soulangeanaSoul的干花蕾中获得不同类型的多酚成分的最高产量。-Bod.var.\'Lennei\'（MSL）。RSM程序中的新颖方法来自UAE条件的同时优化，以获得具有最高抗氧化剂和抗自由基潜力的提取物（作为因变量进行检查），使用适当的分光光度测定法，用Folin-Ciocalteu和2,2-二苯基-1-吡啶酰肼试剂，分别。在55.2分钟的提取方案中使用66.8％（V/V）乙醇作为提取溶剂，提取剂体积与草药物质的比例为46.8mL/g，在所得提取物中生物活性抗氧化剂酚类物质的总收率最高。对于这种草药制剂，使用组合色谱（LC）进行定性和定量分析，光谱(PDA),和串联质谱(ESI-QToF-MS/MS)技术。详细的植物化学分析，第一次进行，记录了大量的各种多酚抗氧化剂，尤其是苯乙醇和类黄酮，在MSL花蕾中。它们的平均总含量超过30.3和36.5毫克/克干重，分别。
