Abstract：
Tetracycline (TC) is a commonly used antibiotic that affects various physiological processes in plants. However, its negative effects on plants remain poorly understood at the molecular level. To ascertain the TC toxicity in the roots, transcriptomic, cytological, and physiological analyses were performed to explore the molecular mechanisms of TC influencing the growth of hulless barley root. At a low concentration (1 mg/L), TC promoted root growth by upregulating the genes related to the flavonoid pathway. At high concentrations (10, 100, and 200 mg/L), TC downregulated genes related to homologous recombination in the root meristem zone and inhibited the mitosis index by 16.4%. Disruption of the DNA repair process can lead to chromosomal aberrations, resulting in a 6.8% C-mitosis rate in the most severe cases. Finally, root growth was inhibited by TC, as evidenced by a reduction in root viability, an increase in reactive oxygen species content, and an inhibition of root length. Cross-comparison of physiological and cytological characterizations and transcriptomic information revealed changes in genetic processes under TC stress. Overall, we present an early genetic strategy to study the significant influence of TC stress on roots.
摘要：
四环素（TC）是一种常用的抗生素，可影响植物的各种生理过程。然而,它对植物的负面影响在分子水平上仍然知之甚少。为了确定根部的TC毒性，转录组，细胞学，进行了生理分析，以探讨TC影响无壳大麦根生长的分子机制。在低浓度（1毫克/升），TC通过上调类黄酮途径相关基因促进根系生长。在高浓度（10、100和200mg/L）下，TC下调与根分生组织区同源重组相关的基因，并抑制有丝分裂指数16.4%。破坏DNA修复过程会导致染色体畸变,在最严重的情况下，C有丝分裂率为6.8％。最后,根系生长受到TC抑制，根活力的降低证明了这一点，活性氧含量的增加，和根长度的抑制。生理和细胞学特征以及转录组信息的交叉比较揭示了TC胁迫下遗传过程的变化。总的来说,我们提出了一种早期遗传策略来研究TC胁迫对根系的显着影响。
