PDF(Pubmed)
Abstract：
Vibriosis is caused by some pathogenic Vibrio and produces significant mortality in Pacific white shrimp Penaeus (Litopenaeus) vannamei larvae in commercial hatcheries. Acute hepatopancreatic necrosis disease (AHPND) is an emerging vibriosis affecting shrimp-producing countries worldwide. Zoea 2 syndrome is another type of vibriosis that affects the early stages of P. vannamei larvae. Although the pathogenesis of AHPND and zoea 2 syndrome is well known, there is scarce information about microbial composition and biomarkers of P.vannamei larvae affected by AHPND, and there is no study of the microbiome of larvae affected by zoea 2 syndrome. In this work, we characterized the microbiome of P. vannamei larvae collected from 12 commercial hatchery tanks by high-throughput sequencing. Seven tanks were affected by AHPND, and five tanks were affected by zoea 2 syndrome. Subsequently, all samples were selected for sequencing of the V3-V4 region of the16S rRNA gene. Similarity analysis using the beta diversity index revealed significant differences in the larval bacterial communities between disease conditions, particularly when Vibrio was analyzed. Linear discriminant analysis with effect size determined specific microbial signatures for AHPND and zoea 2 syndrome. Sneathiella, Cyclobacterium, Haliea, Lewinella, among other genera, were abundant in AHPND-affected larvae. Meanwhile, Vibrio, Spongiimonas, Meridianimaribacter, Tenacibaculum, among other genera, were significantly abundant in larvae affected by zoea 2 syndrome. The bacterial network at the phylum level for larvae collected from tanks affected by AHPND showed greater complexity and connectivity than in samples collected from tanks affected by zoea 2 syndrome. The bacterial connections inter Vibrio genera were higher in larvae from tanks affected by zoea 2 syndrome, also presenting other connections between the genera Vibrio and Catenococcus. The identification of specific biomarkers found in this study could be useful for understanding the microbial dynamics during different types of vibriosis.
摘要：
弧菌病是由某些致病性弧菌引起的，在商业孵化场的太平洋白虾对虾（Litopenaeus）凡纳滨对虾幼虫中产生了显着的死亡率。急性肝胰腺坏死病（AHPND）是一种新兴的弧菌病，影响着全球虾生产国。Zoea2综合征是影响凡纳滨对虾幼虫早期阶段的另一种类型的弧菌病。尽管AHPND和zoea2综合征的发病机制众所周知，关于受AHPND影响的南美白对虾幼虫的微生物组成和生物标志物的信息很少，并且没有研究受zoea2综合征影响的幼虫的微生物组。在这项工作中,我们通过高通量测序对从12个商业孵化场收集的南美白对虾幼虫的微生物组进行了表征。七个坦克受到AHPND的影响，五个坦克受到zoea2综合征的影响。随后，选择所有样本对16SrRNA基因的V3-V4区进行测序.使用β多样性指数进行的相似性分析显示，疾病状况之间的幼虫细菌群落存在显着差异，特别是当弧菌被分析。具有效应大小的线性判别分析确定了AHPND和zoea2综合征的特定微生物特征。Sneathiella,Cyclobacterium,Haliea,Lewinella,在其他属中，在受AHPND影响的幼虫中含量丰富。同时,弧菌,Spongiimonas,子午虫杆菌,肌腱杆菌,在其他属中，在受zoea2综合征影响的幼虫中明显丰富。从受AHPND影响的罐中收集的幼虫在门水平的细菌网络显示出比从受zoea2综合征影响的罐中收集的样品更大的复杂性和连通性。受zoea2综合征影响的坦克幼虫中弧菌属之间的细菌联系较高，还提出了弧菌属和合球菌属之间的其他联系。在这项研究中发现的特定生物标志物的鉴定可能有助于了解不同类型弧菌病期间的微生物动力学。
