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Abstract：
BACKGROUND: Spirometra mansoni can parasitize animals and humans through food and water, causing parasitic zoonosis. Knowledge of the developmental process of S. mansoni is crucial for effective treatment; thus, it is important to characterize differential and specific proteins and pathways associated with parasite development.
METHODS: In this study, we performed a comparative proteomic analysis of the plerocercoid and adult stages using a tandem mass tag-based quantitative proteomic approach. Additionally, integrated transcriptomic and proteomic analyses were conducted to obtain the full protein expression profiles of different life cycle stages of the tapeworm.
RESULTS: Approximately 1166 differentially expressed proteins (DEPs) were identified in adults versus plerocercoids, of which 641 DEPs were upregulated and 525 were downregulated. Gene Ontology (GO), Clusters of Orthologous groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that most DEPs related to genetic information processing and metabolism of energy in adults seem to be more activated. In the plerocercoid stage, compared to metabolism, genetic information processing appears more dynamic. Protein-protein interaction (PPI) revealed six key proteins (phosphomannomutase, glutathione transferase, malate dehydrogenase, cytoplasmic, 40S ribosomal protein S15, ribosomal protein L15 and 60S acidic ribosomal protein P2) that may play active roles in the growth and development of S. mansoni. Finally, the combination of transcriptomic and proteomic data suggested that three pathways (ubiquitin-mediated proteolysis, phagosome and spliceosome) and five proteins closely related to these pathways might have a significant influence in S. mansoni.
CONCLUSIONS: These findings contribute to increasing the knowledge on the protein expression profiles of S. mansoni and provide new insights into functional studies on the molecular mechanisms of the neglected medical tapeworm.
METHODS: In this study, we performed a comparative proteomic analysis of the plerocercoid and adult stages using a tandem mass tag-based quantitative proteomic approach. Additionally, integrated transcriptomic and proteomic analyses were conducted to obtain the full protein expression profiles of different life cycle stages of the tapeworm.
RESULTS: Approximately 1166 differentially expressed proteins (DEPs) were identified in adults versus plerocercoids, of which 641 DEPs were upregulated and 525 were downregulated. Gene Ontology (GO), Clusters of Orthologous groups (COG) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that most DEPs related to genetic information processing and metabolism of energy in adults seem to be more activated. In the plerocercoid stage, compared to metabolism, genetic information processing appears more dynamic. Protein-protein interaction (PPI) revealed six key proteins (phosphomannomutase, glutathione transferase, malate dehydrogenase, cytoplasmic, 40S ribosomal protein S15, ribosomal protein L15 and 60S acidic ribosomal protein P2) that may play active roles in the growth and development of S. mansoni. Finally, the combination of transcriptomic and proteomic data suggested that three pathways (ubiquitin-mediated proteolysis, phagosome and spliceosome) and five proteins closely related to these pathways might have a significant influence in S. mansoni.
CONCLUSIONS: These findings contribute to increasing the knowledge on the protein expression profiles of S. mansoni and provide new insights into functional studies on the molecular mechanisms of the neglected medical tapeworm.
摘要：
背景：Spirometramansoni可以通过食物和水寄生动物和人类，导致寄生虫人畜共患病.了解曼索尼的发育过程对于有效治疗至关重要；因此，表征与寄生虫发育相关的差异和特异性蛋白质和途径是重要的。
方法：在本研究中，我们使用基于串联质量标签的定量蛋白质组学方法对plerocercoid和成体阶段进行了比较蛋白质组学分析.此外,进行了整合的转录组和蛋白质组分析，以获得tape虫不同生命周期阶段的完整蛋白质表达谱。
结果：大约1166个差异表达蛋白（DEP）在成人与plerocercoids中被鉴定，其中641个DEP上调，525个下调。基因本体论（GO），直系同源群（COG）和京都基因和基因组百科全书（KEGG）分析表明，与成人遗传信息处理和能量代谢有关的大多数DEP似乎更激活。在plerocercoid阶段，与新陈代谢相比，遗传信息处理显得更加动态。蛋白质-蛋白质相互作用(PPI)揭示了六个关键蛋白(磷酸甘露聚糖变位酶，谷胱甘肽转移酶，苹果酸脱氢酶，细胞质，40S核糖体蛋白S15，核糖体蛋白L15和60S酸性核糖体蛋白P2）可能在曼氏链球菌的生长发育中起积极作用。最后,转录组和蛋白质组数据的组合表明，三种途径（泛素介导的蛋白水解，吞噬体和剪接体）和与这些途径密切相关的五种蛋白质可能在曼氏链球菌中产生重大影响。
结论：这些发现有助于增加对曼氏球菌蛋白表达谱的了解，并为被忽视的医学tape虫的分子机制的功能研究提供了新的见解。
方法：在本研究中，我们使用基于串联质量标签的定量蛋白质组学方法对plerocercoid和成体阶段进行了比较蛋白质组学分析.此外,进行了整合的转录组和蛋白质组分析，以获得tape虫不同生命周期阶段的完整蛋白质表达谱。
结果：大约1166个差异表达蛋白（DEP）在成人与plerocercoids中被鉴定，其中641个DEP上调，525个下调。基因本体论（GO），直系同源群（COG）和京都基因和基因组百科全书（KEGG）分析表明，与成人遗传信息处理和能量代谢有关的大多数DEP似乎更激活。在plerocercoid阶段，与新陈代谢相比，遗传信息处理显得更加动态。蛋白质-蛋白质相互作用(PPI)揭示了六个关键蛋白(磷酸甘露聚糖变位酶，谷胱甘肽转移酶，苹果酸脱氢酶，细胞质，40S核糖体蛋白S15，核糖体蛋白L15和60S酸性核糖体蛋白P2）可能在曼氏链球菌的生长发育中起积极作用。最后,转录组和蛋白质组数据的组合表明，三种途径（泛素介导的蛋白水解，吞噬体和剪接体）和与这些途径密切相关的五种蛋白质可能在曼氏链球菌中产生重大影响。
结论：这些发现有助于增加对曼氏球菌蛋白表达谱的了解，并为被忽视的医学tape虫的分子机制的功能研究提供了新的见解。
