PDF(Pubmed)
Abstract：
Picobirnaviruses (PBVs) are double-stranded RNA viruses frequently detected in human and animal enteric viromes. Associations of PBVs with enteric graft-versus-host disease and type I diabetes during pregnancy have been established. Since their discovery in 1988, PBVs have been generally assumed to be animal-infecting viruses despite the lack of culture system, animal model, or detection in animal cells or tissues. Recent studies have proposed that bacteria or fungi could be the hosts of PBVs based on genomic analysis. Here, we functionally demonstrate that multiple PBVs of different genome organizations encode bacterial lysins that lyse Escherichia coli. Such genes are typically encoded only by bacteriophages supporting the model that PBVs infect bacterial hosts. Recognition of PBVs as RNA phages in the human gut would completely shift models of how PBVs could impact human health. In addition, expanding the RNA phage world beyond the two recognized clades to three clades has implications for our understanding of the evolution of RNA viruses.
摘要：
picobirnairvirus(PBVs)是在人和动物肠道病毒中经常检测到的双链RNA病毒。已经建立了PBV与妊娠期间肠移植物抗宿主病和I型糖尿病的关联。自1988年发现PBV以来，尽管缺乏培养系统，但通常被认为是动物感染病毒，动物模型,或在动物细胞或组织中检测。最近的研究表明，基于基因组分析，细菌或真菌可能是PBV的宿主。这里,我们在功能上证明，不同基因组组织的多个PBV编码裂解大肠杆菌的细菌溶素。此类基因通常仅由支持PBV感染细菌宿主的模型的噬菌体编码。将PBV识别为人类肠道中的RNA噬菌体将完全改变PBV如何影响人类健康的模型。此外,将RNA噬菌体世界从两个公认的进化枝扩展到三个进化枝，这对我们理解RNA病毒的进化具有重要意义。
