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Abstract：
UNASSIGNED: Keloids are excessive fibroproliferative diseases that are caused by abnormal wound healing. The anti-proliferative activity of Physalis angulata compounds has potential as a keloid therapeutic agent. This study aimed to observe the effects of P. angulata on fibroblast viability and collagen type I, tissue inhibitor of metalloproteinase 1 (TIMP-1), and plasminogen activator inhibitor 1 (PAI-1) levels in human keloid fibroblasts.
UNASSIGNED: We conducted an experimental study of P. angulata ethanol extract on three primary human keloid fibroblast 3 passage cultures with four replications. Fibroblast viability was measured using the MTT assay after incubation with 3, 5, and 10 µg/mL P. angulata. Concentrations of P. angulata used to observe effects on TIMP-1, PAI-1, and collagen type I levels were 10%, 20%, 30%, and 40% of inhibitory concentration 50 (IC50). The levels of collagen type I, TIMP-1, and PAI-1 were measured by ELISA. Mean comparisons between multiple treatment groups were analyzed using one-way ANOVA followed by post-hoc analysis.
UNASSIGNED: The 10 µg/mL P. angulata group had significantly lower fibroblast viability than the control group (p<0.05) with an IC50 6.3 µg/mL. The collagen type I level of 10% IC50 (0.63 µg/mL) P. angulata group was lower than control (12.910 vs 47.866 ng/mL) (p=0.042). Level of TIMP-1 in 40% IC50 (2.51 µg/mL) P. angulata group was lower than control (5.350 vs 9.972 ng/mL) (p=0.043). There was no significant difference in the PAI-1 levels.
UNASSIGNED: This study showed the inhibitory effect of 10 µg/mL P. angulata extract on keloid fibroblast viability, with an IC50 of 6.3 µg/mL. This study also showed collagen type-1 and TIMP-1 inhibition, but not PAI-1 inhibition, after P. angulate treatment.
UNASSIGNED: We conducted an experimental study of P. angulata ethanol extract on three primary human keloid fibroblast 3 passage cultures with four replications. Fibroblast viability was measured using the MTT assay after incubation with 3, 5, and 10 µg/mL P. angulata. Concentrations of P. angulata used to observe effects on TIMP-1, PAI-1, and collagen type I levels were 10%, 20%, 30%, and 40% of inhibitory concentration 50 (IC50). The levels of collagen type I, TIMP-1, and PAI-1 were measured by ELISA. Mean comparisons between multiple treatment groups were analyzed using one-way ANOVA followed by post-hoc analysis.
UNASSIGNED: The 10 µg/mL P. angulata group had significantly lower fibroblast viability than the control group (p<0.05) with an IC50 6.3 µg/mL. The collagen type I level of 10% IC50 (0.63 µg/mL) P. angulata group was lower than control (12.910 vs 47.866 ng/mL) (p=0.042). Level of TIMP-1 in 40% IC50 (2.51 µg/mL) P. angulata group was lower than control (5.350 vs 9.972 ng/mL) (p=0.043). There was no significant difference in the PAI-1 levels.
UNASSIGNED: This study showed the inhibitory effect of 10 µg/mL P. angulata extract on keloid fibroblast viability, with an IC50 of 6.3 µg/mL. This study also showed collagen type-1 and TIMP-1 inhibition, but not PAI-1 inhibition, after P. angulate treatment.
摘要：
■瘢痕疙瘩是由异常伤口愈合引起的过度纤维增生性疾病。酸浆化合物的抗增殖活性具有作为瘢痕疙瘩治疗剂的潜力。本研究旨在观察泡斑对成纤维细胞活力和Ⅰ型胶原的影响。金属蛋白酶组织抑制剂1（TIMP-1），和纤溶酶原激活物抑制剂1（PAI-1）在人瘢痕疙瘩成纤维细胞中的水平。
■我们对三种原代人瘢痕疙瘩成纤维细胞3传代培养物进行了实验研究，并进行了四次重复。在与3、5和10μg/mL棱角假单胞菌孵育后，使用MTT测定法测量成纤维细胞活力。用于观察对TIMP-1，PAI-1和I型胶原蛋白水平的影响的棱柱体的浓度为10％，20%,30%,和40％的抑制浓度50（IC50）。I型胶原蛋白的水平，通过ELISA测量TIMP-1和PAI-1。使用单向ANOVA和事后分析分析多个治疗组之间的平均比较。
■与对照组相比，10µg/mL角斗鱼组的成纤维细胞活力显着降低（p<0.05），IC50为6.3µg/mL。10％IC50（0.63µg/mL）的I型胶原水平。棱柱体组低于对照组（12.910比47.866ng/mL）（p=0.042）。40％IC50（2.51µg/mL）的TIMP-1水平。棱柱体组低于对照组（5.350对9.972ng/mL）（p=0.043）。PAI-1水平无显著差异。
■这项研究显示了10µg/mL的角斗鱼提取物对瘢痕疙瘩成纤维细胞活力的抑制作用，IC50为6.3µg/mL。这项研究还显示了胶原蛋白1型和TIMP-1的抑制作用，但不是PAI-1抑制，在P.成角度治疗后。
■我们对三种原代人瘢痕疙瘩成纤维细胞3传代培养物进行了实验研究，并进行了四次重复。在与3、5和10μg/mL棱角假单胞菌孵育后，使用MTT测定法测量成纤维细胞活力。用于观察对TIMP-1，PAI-1和I型胶原蛋白水平的影响的棱柱体的浓度为10％，20%,30%,和40％的抑制浓度50（IC50）。I型胶原蛋白的水平，通过ELISA测量TIMP-1和PAI-1。使用单向ANOVA和事后分析分析多个治疗组之间的平均比较。
■与对照组相比，10µg/mL角斗鱼组的成纤维细胞活力显着降低（p<0.05），IC50为6.3µg/mL。10％IC50（0.63µg/mL）的I型胶原水平。棱柱体组低于对照组（12.910比47.866ng/mL）（p=0.042）。40％IC50（2.51µg/mL）的TIMP-1水平。棱柱体组低于对照组（5.350对9.972ng/mL）（p=0.043）。PAI-1水平无显著差异。
■这项研究显示了10µg/mL的角斗鱼提取物对瘢痕疙瘩成纤维细胞活力的抑制作用，IC50为6.3µg/mL。这项研究还显示了胶原蛋白1型和TIMP-1的抑制作用，但不是PAI-1抑制，在P.成角度治疗后。
