Abstract：
OBJECTIVE: To evaluate the effect of progesterone use on fetal fraction (FF) in non-invasive prenatal testing (NIPT) due to the threat of first trimester miscarriage.
METHODS: This case control study included the pregnant who were referred to our clinic for non-invasive prenatal testing. The patients were categorized into three groups: Pregnant women with vaginal bleeding and using progesterone, pregnant women with vaginal bleeding and not using progesterone, and pregnant women without bleeding. The groups were formed by matching gestational week. Women with multiple pregnancy, BMI (body mass index) ≥25, abnormal fetal karyotype, and chronic disease were excluded from the study. Maternal characteristics, FF of the NIPT were recruited from the computer based medical records.
RESULTS: A total of 10,275 NIPT tests were performed during the study period. 3% of the patients (n = 308) were found at risk of miscarriage. 100 patients with a vaginal bleeding and 50 control patients were matched. The median value of the fetal fraction ratio was found to be 6.55 in pregnant women without vaginal bleeding, 7.05 in pregnant women who had vaginal bleeding and using progesterone, and 7.3 in pregnant women who had vaginal bleeding and did not use progesterone. Although the fetal fraction ratio was found to be higher in pregnant women with vaginal bleeding and lower in progesterone users, this situation could not reach the level of statistical significance (p = 0.351).
CONCLUSIONS: The fetal fraction rate in maternal blood is not affected in pregnant women who use progesterone due to vaginal bleeding in early gestational weeks.
METHODS: This case control study included the pregnant who were referred to our clinic for non-invasive prenatal testing. The patients were categorized into three groups: Pregnant women with vaginal bleeding and using progesterone, pregnant women with vaginal bleeding and not using progesterone, and pregnant women without bleeding. The groups were formed by matching gestational week. Women with multiple pregnancy, BMI (body mass index) ≥25, abnormal fetal karyotype, and chronic disease were excluded from the study. Maternal characteristics, FF of the NIPT were recruited from the computer based medical records.
RESULTS: A total of 10,275 NIPT tests were performed during the study period. 3% of the patients (n = 308) were found at risk of miscarriage. 100 patients with a vaginal bleeding and 50 control patients were matched. The median value of the fetal fraction ratio was found to be 6.55 in pregnant women without vaginal bleeding, 7.05 in pregnant women who had vaginal bleeding and using progesterone, and 7.3 in pregnant women who had vaginal bleeding and did not use progesterone. Although the fetal fraction ratio was found to be higher in pregnant women with vaginal bleeding and lower in progesterone users, this situation could not reach the level of statistical significance (p = 0.351).
CONCLUSIONS: The fetal fraction rate in maternal blood is not affected in pregnant women who use progesterone due to vaginal bleeding in early gestational weeks.
摘要：
目的：评估由于妊娠早期流产的威胁，在非侵入性产前检测（NIPT）中使用孕酮对胎儿分数（FF）的影响。
方法：本病例对照研究包括被转诊到我们诊所进行非侵入性产前检测的孕妇。患者分为三组：孕妇阴道出血和使用孕酮，孕妇阴道出血和不使用黄体酮，孕妇没有出血.各组由匹配的孕周组成。多胎妊娠妇女,BMI（体重指数）≥25，胎儿核型异常，和慢性疾病被排除在研究之外。产妇特征,从基于计算机的医疗记录中招募NIPT的FF。
结果：在研究期间共进行了10,275项NIPT测试。3％的患者（n=308）有流产的风险。匹配100例阴道出血患者和50例对照患者。在没有阴道出血的孕妇中发现胎儿分数比率的中位数为6.55,7.05在阴道出血和使用孕酮的孕妇中，阴道出血且未使用孕酮的孕妇为7.3。尽管发现阴道出血的孕妇的胎儿分数比率较高，而孕酮使用者的胎儿分数较低，这种情况无法达到统计学意义的水平（p=0.351）。
结论：在孕周早期因阴道出血而使用孕酮的孕妇中，母体血液中的胎儿分数不受影响。
方法：本病例对照研究包括被转诊到我们诊所进行非侵入性产前检测的孕妇。患者分为三组：孕妇阴道出血和使用孕酮，孕妇阴道出血和不使用黄体酮，孕妇没有出血.各组由匹配的孕周组成。多胎妊娠妇女,BMI（体重指数）≥25，胎儿核型异常，和慢性疾病被排除在研究之外。产妇特征,从基于计算机的医疗记录中招募NIPT的FF。
结果：在研究期间共进行了10,275项NIPT测试。3％的患者（n=308）有流产的风险。匹配100例阴道出血患者和50例对照患者。在没有阴道出血的孕妇中发现胎儿分数比率的中位数为6.55,7.05在阴道出血和使用孕酮的孕妇中，阴道出血且未使用孕酮的孕妇为7.3。尽管发现阴道出血的孕妇的胎儿分数比率较高，而孕酮使用者的胎儿分数较低，这种情况无法达到统计学意义的水平（p=0.351）。
结论：在孕周早期因阴道出血而使用孕酮的孕妇中，母体血液中的胎儿分数不受影响。
