Abstract：
HIV-1CRF08_BC is the most prevalent epidemic subtype among heterosexual (HET) and intravenous drug users (IDUs) in Kunming, Yunnan. Using the pol region of gene sequences derived from molecular epidemiological surveys, we developed a molecular transmission network for the purpose of analyzing its epidemiological characteristics, assessing its epidemiological trends, identifying its potential transmission relationships, and developing targeted interventions. HyPhy 2.2.4 was used to calculate pairwise genetic distances between sequences; GraphPad-Prism 8.0 was employed to determine the standard genetic distance; and Cytoscope 3.7.2 was applied to visualize the network. We used the network analysis tools to investigate network characteristics and the Molecular Complex Detection (MCODE) tool to observe the growth of the network. We utilized a logistic regression model to examine the factors influencing clustering and a zero-inflated Poisson model to investigate the factors influencing potential transmission links. At the standard genetic distance threshold of 0.008, 406 out of 858 study participants were clustered in 132 dissemination networks with a total network linkage of 868, and the number of links per sequence ranged from 1 to 19. The MCODE analysis identified three significant modular clusters in the networks, with network scores ranging from 4.9 to 7. In models of logistic regression, HET, middle-aged and elderly individuals, and residents of northern and southeastern Kunming were more likely to enter the transmission network. According to the zero-inflated Poisson model, age, transmission category, sampling year, marital status, and CD4+ T level had a significant effect on the size of links. The molecular clusters in Kunming\'s molecular transmission network are specific and aggregate to a certain extent. HIV-1 molecular network analysis provided information on local transmission characteristics, and these findings helped to determine the priority of transmission-reduction interventions.
摘要：
背景HIV-1CRF08_BC是昆明异性恋（HET）和静脉吸毒者（IDUs）中最普遍的流行亚型，云南。利用来自分子流行病学调查的基因序列的pol区域，我们开发了一个分子传输网络来分析其流行病学特征，评估其流行病学趋势，确定其潜在的传播关系，制定有针对性的干预措施。方法使用HyPhy2.2.4计算序列之间的成对遗传距离；使用GraphPad-Prism8.0确定标准遗传距离；应用细胞镜3.7.2对网络进行可视化。使用网络分析工具调查网络特征，使用MCODE工具观察网络的增长。我们使用逻辑回归模型来检查影响聚类的因素，并使用零膨胀泊松模型来研究影响潜在传输链接的因素。结果在0.008的标准遗传距离阈值下，858名研究参与者中有406人聚集在132个传播网络中，总网络链接为868个，每个序列的链接数在1到19之间。MCODE分析确定了网络中的三个重要的模块化集群，网络分数从4.9到7。在逻辑回归模型中，HET,中老年人,昆明北部和东南部的居民更容易进入传输网络（均P<0.05）。根据零膨胀泊松模型,年龄,变速器类别,采样年，婚姻状况,CD4+T水平对链接的大小有显著影响(均P<0.05)。结论昆明分子传输网络中的分子簇具有一定的特异性和聚集性。HIV-1分子网络分析提供了有关局部传播特征的信息，这些发现有助于确定减少传播干预措施的优先级。
