RESULTS: Seventy-six indel loci of 11 genes in JAK/STAT were detected, and three genotypes were selected at four loci by PCR amplification, electrophoresis and sequencing, including one locus in STAT3, one locus in STAT5A, and two loci in JAK1. The Correlation analysis indicated that there was no significant correlation between STAT3 and growth traits in four sheep breeds (P > 0.05); STAT5A was significantly associated with body height, rump width and tube circumference in Hu sheep and body length in Tong sheep (P < 0.05); JAK1 was significantly correlated with body height, body oblique length, cross height and tube circumference in Hu sheep (P < 0.05) and body oblique length, cross height and tube circumference in small-tailed Han sheep (P < 0.05).
CONCLUSIONS: Overall, our results indicated a potential association between the growth traits of sheep and the InDels of JAK1 and STAT5A.
结果:在JAK/STAT中检测到11个基因的76个indel位点,通过PCR扩增在四个基因座上选择三个基因型,电泳和测序,包括STAT3中的一个基因座,STAT5A中的一个基因座,和JAK1中的两个基因座。相关分析表明,4个绵羊品种的STAT3与生长性状无显著相关性(P>0.05);STAT5A与身高呈显著相关,湖羊的臀部宽度和管围以及童羊的体长(P<0.05);JAK1与身高显着相关,身体倾斜长度,湖羊的交叉高度和管围(P<0.05)和身体斜长,小尾寒羊横高和管围(P<0.05)。
结论:总体而言,我们的结果表明,绵羊的生长性状与JAK1和STAT5A的InDels之间存在潜在的关联。