Abstract：
Erythromycin, a commonly used macrolide antibiotic, plays a crucial role in both human medicine and animal husbandry. However, its abuse has led to residual presence in the environment, with problems such as the emergence of resistant bacteria and enrichment of resistance genes. These issues pose significant risks to human health. Thus far, there are no effective, environmentally friendly methods to manage this problem. Enzymes can specifically degrade erythromycin without causing other problems, but their unrecyclability and environmental vulnerability hinder large-scale application. Enzyme immobilization may help to solve these problems. This study used Cu-BTC, a synthetic metal-organic framework, to immobilize the erythromycin-degrading enzyme EreB. The loading temperature and enzyme quantity were optimized. The Cu-BTC and EreB@Cu-BTC were characterized by various methods to confirm the preparation of Cu-BTC and immobilization of EreB. The maximum enzyme loading capacity was 66.5 mg g-1. In terms of enzymatic properties, immobilized EreB had improved heat (25-45 °C) and alkaline (6.5-10) tolerance, along with greater affinity between the enzyme and its substrate; Km decreased from 438.49 to 372.30 mM. Recycling was also achieved; after 10 cycles, 57.12% of the enzyme activity was maintained. After composite degradation, the antibacterial activity of erythromycin-containing wastewater was examined; the results showed that the novel composite could completely inactivate erythromycin. In summary, Cu-BTC was an ideal carrier for immobilization of the enzyme EreB, and the EreB@Cu-BTC composite has good prospects for the treatment of erythromycin-containing wastewater.
摘要：
红霉素,一种常用的大环内酯抗生素，在人类医学和畜牧业中起着至关重要的作用。然而,它的滥用导致了环境中的残余存在，具有抗性细菌的出现和抗性基因的富集等问题。这些问题对人类健康构成重大风险。到目前为止,没有有效的，解决这个问题的环保方法。酶可以特异性降解红霉素而不会引起其他问题，但是它们的不可回收性和环境脆弱性阻碍了大规模应用。酶固定化可能有助于解决这些问题。本研究使用Cu-BTC，合成的金属有机框架，固定红霉素降解酶EreB。对上样温度和酶量进行了优化。通过各种方法对Cu-BTC和EreB@Cu-BTC进行表征，以确定Cu-BTC的制备和EreB的固定化。最大酶负载能力为66.5mgg-1。就酶学性质而言，固定化EreB具有改善的热(25-45°C)和碱性(6.5-10)耐受性，随着酶与其底物之间更大的亲和力；Km从438.49降低到372.30mM。回收也实现了；经过10个循环，保持了57.12%的酶活性。复合材料降解后，研究了含红霉素废水的抗菌活性；结果表明，新型复合材料可以完全灭活红霉素。总之,Cu-BTC是固定化酶EreB的理想载体,EreB@Cu-BTC复合材料在含红霉素废水的处理中具有良好的前景。
