Abstract：
Lifestyle factors, including smoking, have been linked to neoplastic diseases, and reports suggest an association between smoking and overexpression of FGFR (fibroblast growth factor receptor) in certain neoplasms. This study aims to assess the expression of FGFR3 and FGFR4 genes in patients with and without a history of smoking.A total of 118 participants were recruited, including 83 Juvenile Nasopharyngeal Angiofibroma (JNA) patients and 35 healthy participants, the JNA patients were further stratified as smokers and nonsmokers. Total RNA was extracted from the blood & saliva sample by using TRIzol reagent, and quantified using a Nanodrop, and then subjected to gene expression analysis of FGFR3/4 using RT-PCR. Immunohistochemistry analysis was employed using fresh biopsies of JNA to validate the findings. All experiments were performed in triplicates and analysed using the Chi-Square test (P < 0.05). Smokers exhibited significantly lower total RNA concentrations across all sample types (P < 0.001). The study revealed significant upregulation of both FGFR3/4 genes in JNA patients (P < 0.05). Moreover, FGFR3 expression was significantly higher among smokers 66% (95% CI: 53-79%) compared to non-smokers 22% (95% CI: 18-26%). Immunohistochemistry analysis demonstrated moderate to strong staining intensity for FGFR3 among smokers. The study highlights the overexpression of FGFR3/4 genes in JNA patients, with a stronger association observed among smokers. Furthermore, medical reports indicated higher rates of recurrence and bleeding intensity among smokers. These findings emphasize the potential role of FGFR3 as a key molecular factor in JNA, particularly in the context of smoking.
摘要：
生活方式因素,包括吸烟,与肿瘤疾病有关，报道提示吸烟与某些肿瘤中FGFR(成纤维细胞生长因子受体)的过度表达之间存在关联。这项研究旨在评估有和没有吸烟史的患者FGFR3和FGFR4基因的表达。总共招募了118名参与者，包括83名青少年鼻咽血管纤维瘤（JNA）患者和35名健康参与者，将JNA患者进一步分层为吸烟者和非吸烟者.使用TRIzol试剂从血液和唾液样品中提取总RNA，并使用Nanodrop进行量化，然后使用RT-PCR对FGFR3/4进行基因表达分析。使用JNA的新鲜活检采用免疫组织化学分析来验证发现。所有实验一式三份进行,并使用卡方检验进行分析(P<0.05)。吸烟者在所有样品类型中表现出显著较低的总RNA浓度(P<0.001)。研究显示JNA患者中两种FGFR3/4基因均显著上调(P<0.05)。此外，FGFR3表达在吸烟者中显著高于66%(95%CI:53-79%),而非吸烟者为22%(95%CI:18-26%)。免疫组织化学分析表明吸烟者中FGFR3的中等至强染色强度。该研究强调了JNA患者中FGFR3/4基因的过度表达，在吸烟者中观察到更强的关联。此外，医学报告显示吸烟者的复发率和出血强度较高.这些发现强调了FGFR3作为JNA关键分子因子的潜在作用，特别是在吸烟的背景下。
