Abstract：
Diet is one of the main factors that modifies intestinal microbiota composition. The search for foods that can reverse situations of intestinal dysbiosis such as that induced by antibiotics is of great interest. Buttermilk and whey are the main by-products produced by the dairy industry containing bioactive compounds. The aim of this study is to investigate the ability of whey and buttermilk-based formulas supplemented with lactoferrin and milk fat globule membrane (MFGM) to modulate the effects of clindamycin on mouse intestinal microbiota.
Male C57BL/6 mice are treated with saline (control), clindamycin (Clin), a formula containing whey (F1) or buttermilk (F2), Clin+F1 or Clin+F2, and their fecal microbiota profiles are analyzed by sequencing of 16S rRNA gene using the MinION device. Clin induces alterations in both the composition and metabolic functions of the mice intestinal microbiota. The treatment with F1 or F2 reverses the effects of clindamycin, restoring the levels of Rikenellaceae and Lactobacillaceae families and certain pathways related to short-chain fatty acids production and tetrahydrofolate biosynthesis.
Whey and buttermilk supplemented with lactoferrin and MFGM may be a bioactive formula for functional foods to prevent or restore microbiota alterations induced by antibiotic administration.
Male C57BL/6 mice are treated with saline (control), clindamycin (Clin), a formula containing whey (F1) or buttermilk (F2), Clin+F1 or Clin+F2, and their fecal microbiota profiles are analyzed by sequencing of 16S rRNA gene using the MinION device. Clin induces alterations in both the composition and metabolic functions of the mice intestinal microbiota. The treatment with F1 or F2 reverses the effects of clindamycin, restoring the levels of Rikenellaceae and Lactobacillaceae families and certain pathways related to short-chain fatty acids production and tetrahydrofolate biosynthesis.
Whey and buttermilk supplemented with lactoferrin and MFGM may be a bioactive formula for functional foods to prevent or restore microbiota alterations induced by antibiotic administration.
摘要：
方法：饮食是改变肠道菌群组成的主要因素之一。寻找可以逆转肠道生态失调的情况(例如由抗生素引起的情况)的食物是非常感兴趣的。Buttermilk和乳清是乳制品行业产生的主要副产品，含有生物活性化合物。这项研究的目的是研究补充乳铁蛋白和乳脂球膜（MFGM）的基于乳清和酪乳的配方调节克林霉素对小鼠肠道微生物群的影响的能力。
结果：雄性C57BL/6小鼠用盐水处理（对照），克林霉素（Clin），含有乳清(F1)或酪乳(F2)的配方，通过使用MinION装置对16SrRNA基因进行测序来分析Clin+F1或Clin+F2及其粪便微生物区系谱。Clin诱导小鼠肠道微生物群的组成和代谢功能的改变。用F1或F2治疗可以逆转克林霉素的作用，恢复Rikenellaceae和乳杆菌科的水平以及与短链脂肪酸生产和四氢叶酸生物合成相关的某些途径。
结论：补充乳铁蛋白和MFGM的乳清和酪乳可能是功能性食品的生物活性配方，以预防或恢复抗生素给药引起的微生物群改变。
结果：雄性C57BL/6小鼠用盐水处理（对照），克林霉素（Clin），含有乳清(F1)或酪乳(F2)的配方，通过使用MinION装置对16SrRNA基因进行测序来分析Clin+F1或Clin+F2及其粪便微生物区系谱。Clin诱导小鼠肠道微生物群的组成和代谢功能的改变。用F1或F2治疗可以逆转克林霉素的作用，恢复Rikenellaceae和乳杆菌科的水平以及与短链脂肪酸生产和四氢叶酸生物合成相关的某些途径。
结论：补充乳铁蛋白和MFGM的乳清和酪乳可能是功能性食品的生物活性配方，以预防或恢复抗生素给药引起的微生物群改变。
