Abstract：
BACKGROUND: Histology of human oral mucosa is closely related with its function and anatomical location, and a proper characterization of the human masticatory oral mucosa could be very useful in periodontal pathology.
OBJECTIVE: In the present work, we have carried out a comprehensive study in order to determine the main histological features of parakeratinized (POM) and orthokeratinized (OOM) masticatory human oral mucosa using light and electron microscopy.
METHODS: To perform this, we have used several histological, histochemical and immunohistochemical methods to detect key markets at the epithelial, basement membrane and connective tissue levels.
RESULTS: Our results demonstrated that POM and OOM share many histological similarities, as expected. However, important differences were observed at the epithelial layer of POM, that was significantly thicker than the epithelial layer found in OOM, especially due to a higher number of cells at the stratum spinosum. The expression pattern of CK10 and filaggrin revealed intense signal expression in OOM as compared to POM. Collagen and proteoglycans were more abundant in OOM stroma than in POM. No differences were found for blood vessels and basement membrane.
CONCLUSIONS: These results may contribute to a better understanding of the pathological conditions affecting the human masticatory oral mucosa. In addition, these findings could be useful for the generation of different types of oral mucosa by tissue engineering techniques.
UNASSIGNED: Microscopical features of parakeratinized and orthokeratinized masticatory human oral mucosa showed important differences at both, epithelial and stromal levels. Parakeratinized masticatory human oral mucosa exert thicker epithelial layer, especially, at the stratum spinosum in comparison to orthokeratinized human oral mucosa. Cytokeratin 10 and filaggrin human epithelial markers were intensively expressed in orthokeratinized masticatory human oral mucosa in comparison to parakeratinized masticatory human oral mucosa. At the stromal level, orthokeratinized masticatory human oral mucosa exhibit higher levels of collagen and proteoglycans than parakeratinized masticatory oral mucosa. The deep knowledge of histological features of masticatory oral mucosa could lead to a better understanding of oral mucosa pathology and advanced treatments.
OBJECTIVE: In the present work, we have carried out a comprehensive study in order to determine the main histological features of parakeratinized (POM) and orthokeratinized (OOM) masticatory human oral mucosa using light and electron microscopy.
METHODS: To perform this, we have used several histological, histochemical and immunohistochemical methods to detect key markets at the epithelial, basement membrane and connective tissue levels.
RESULTS: Our results demonstrated that POM and OOM share many histological similarities, as expected. However, important differences were observed at the epithelial layer of POM, that was significantly thicker than the epithelial layer found in OOM, especially due to a higher number of cells at the stratum spinosum. The expression pattern of CK10 and filaggrin revealed intense signal expression in OOM as compared to POM. Collagen and proteoglycans were more abundant in OOM stroma than in POM. No differences were found for blood vessels and basement membrane.
CONCLUSIONS: These results may contribute to a better understanding of the pathological conditions affecting the human masticatory oral mucosa. In addition, these findings could be useful for the generation of different types of oral mucosa by tissue engineering techniques.
UNASSIGNED: Microscopical features of parakeratinized and orthokeratinized masticatory human oral mucosa showed important differences at both, epithelial and stromal levels. Parakeratinized masticatory human oral mucosa exert thicker epithelial layer, especially, at the stratum spinosum in comparison to orthokeratinized human oral mucosa. Cytokeratin 10 and filaggrin human epithelial markers were intensively expressed in orthokeratinized masticatory human oral mucosa in comparison to parakeratinized masticatory human oral mucosa. At the stromal level, orthokeratinized masticatory human oral mucosa exhibit higher levels of collagen and proteoglycans than parakeratinized masticatory oral mucosa. The deep knowledge of histological features of masticatory oral mucosa could lead to a better understanding of oral mucosa pathology and advanced treatments.
摘要：
背景：人类口腔粘膜的组织学与功能和解剖位置密切相关，正确表征人类咀嚼口腔粘膜可能对牙周病理学非常有用。
目的：在目前的工作中，我们进行了一项全面的研究，以确定使用光学和电子显微镜检查的偏角化（POM）和正交化（OOM）咀嚼人口腔粘膜的主要组织学特征。
方法：要执行此操作，我们使用了几种组织学，组织化学和免疫组织化学方法来检测上皮的主要市场，基底膜和结缔组织水平。
结果：我们的结果表明POM和OOM具有许多组织学相似性，如预期。然而,在POM的上皮层观察到重要的差异，明显比OOM中的上皮层厚，特别是由于棘层的细胞数量较多。与POM相比，CK10和聚丝蛋白的表达模式揭示了OOM中强烈的信号表达。OOM基质中的胶原蛋白和蛋白聚糖比POM中的更丰富。血管和基底膜没有发现差异。
结论：这些结果可能有助于更好地了解影响人类咀嚼性口腔粘膜的病理状况。此外,这些发现可能有助于通过组织工程技术产生不同类型的口腔粘膜。
■人咀嚼性口腔黏膜的显微镜特征显示出两者的重要差异，上皮和基质水平。假性咀嚼人口腔粘膜发挥较厚的上皮层，尤其是,与角化的人口腔粘膜相比，在棘层。与角化咀嚼人类口腔粘膜相比，细胞角蛋白10和聚丝团蛋白人类上皮标志物在正交咀嚼人类口腔粘膜中强烈表达。在基质层面，正交咀嚼的人口腔黏膜比半咀嚼的口腔黏膜表现出更高的胶原蛋白和蛋白聚糖水平。对咀嚼性口腔黏膜组织学特征的深入了解可以使人们更好地了解口腔黏膜病理和晚期治疗方法。
目的：在目前的工作中，我们进行了一项全面的研究，以确定使用光学和电子显微镜检查的偏角化（POM）和正交化（OOM）咀嚼人口腔粘膜的主要组织学特征。
方法：要执行此操作，我们使用了几种组织学，组织化学和免疫组织化学方法来检测上皮的主要市场，基底膜和结缔组织水平。
结果：我们的结果表明POM和OOM具有许多组织学相似性，如预期。然而,在POM的上皮层观察到重要的差异，明显比OOM中的上皮层厚，特别是由于棘层的细胞数量较多。与POM相比，CK10和聚丝蛋白的表达模式揭示了OOM中强烈的信号表达。OOM基质中的胶原蛋白和蛋白聚糖比POM中的更丰富。血管和基底膜没有发现差异。
结论：这些结果可能有助于更好地了解影响人类咀嚼性口腔粘膜的病理状况。此外,这些发现可能有助于通过组织工程技术产生不同类型的口腔粘膜。
■人咀嚼性口腔黏膜的显微镜特征显示出两者的重要差异，上皮和基质水平。假性咀嚼人口腔粘膜发挥较厚的上皮层，尤其是,与角化的人口腔粘膜相比，在棘层。与角化咀嚼人类口腔粘膜相比，细胞角蛋白10和聚丝团蛋白人类上皮标志物在正交咀嚼人类口腔粘膜中强烈表达。在基质层面，正交咀嚼的人口腔黏膜比半咀嚼的口腔黏膜表现出更高的胶原蛋白和蛋白聚糖水平。对咀嚼性口腔黏膜组织学特征的深入了解可以使人们更好地了解口腔黏膜病理和晚期治疗方法。
