Abstract：
Although epithelial-mesenchymal markers play an important role in prostate cancer (PC), further research is needed to better understand their utility in diagnosis, cancer progression prevention, and treatment resistance prediction. Our study included 111 PC patients who underwent transurethral resection, as well as 16 healthy controls. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to examine the expression of E-cadherin, β-catenin, and Vimentin. We found that E-cadherin and β-catenin were underexpressed in primary PC tissues. E-cadherin expression was found to be inversely associated with prostate-specific antigen progression (PSA-P; serum marker of progression; p = 0.01; |r| = 0.262). Furthermore, the underexpression of two markers, E-cadherin and β-catenin, was found to be associated with advanced tumor stage and grade (p < 0.05). On the other hand, Vimentin was overexpressed in PC patients with a fold change of 2.141, and it was associated with the diagnosis, prognosis, and prediction of treatment resistance to androgen deprivation therapy (p = 0.002), abiraterone-acid (p = 0.001), and taxanes (p = 0.029). Moreover, the current study highlighted that poor survival could be significantly found in patients who progressed after primary surgery, did not use drugs, and expressed these genes aberrantly. In Cox regression multivariate analysis (p < 0.05), a positive correlation between the Vimentin marker and coronary heart disease in PC patients was identified (p = 0.034). In summary, the present study highlights the diagnostic (p < 0.001), prognostic (p < 0.001), and therapeutic potential of Vimentin in primary PC (p < 0.05), as well as its implications for cardiovascular disease. Furthermore, we confirm the potential prognostic value of E-cadherin and β-catenin.
摘要：
尽管上皮-间质标志物在前列腺癌（PC）中起重要作用，需要进一步的研究来更好地了解它们在诊断中的效用，癌症进展预防,和治疗抗性预测。我们的研究包括111例经尿道电切术的PC患者，以及16个健康对照。逆转录-定量聚合酶链反应(RT-qPCR)用于检测E-cadherin的表达,β-连环蛋白，还有Vimentin.我们发现E-cadherin和β-catenin在原发性PC组织中表达不足。发现E-cadherin表达与前列腺特异性抗原进展呈负相关(PSA-P;进展的血清标志物;p=0.01;|r|=0.262)。此外，两个标记的减压，E-钙粘蛋白和β-连环蛋白，发现与晚期肿瘤分期和分级相关(p<0.05)。另一方面,波形蛋白在PC患者中过度表达，倍数变化为2.141，与诊断相关，预后,以及对雄激素剥夺疗法的治疗抗性的预测（p=0.002），阿比特龙-酸（p=0.001），和紫杉烷（p=0.029）。此外，目前的研究强调，在初次手术后进展的患者中，生存率明显下降，没有使用药物，并异常表达了这些基因。在Cox回归多变量分析中(p<0.05)，在PC患者中,Vimentin标志物与冠心病呈正相关(p=0.034).总之,本研究强调了诊断性(p<0.001)，预后（p<0.001），波形蛋白在原发性PC中的治疗潜力（p<0.05），以及它对心血管疾病的影响。此外，我们证实了E-cadherin和β-catenin的潜在预后价值。
