PDF(Pubmed)
Abstract：
UNASSIGNED: The temporomandibular joint (TMJ) is a synovial joint that allows the complex movements essential for life. It connects the jawbone to the skull, working as a sliding hinge. Moreover, pluripotent stem cells are a source of precursors and tissue-specific cells in developing organisms, however, their biodistribution in developing fetal tissues is weakly studied. The aim of our study was analyse immunohistochemical expression of Nanog, Oct-4, Sox-2 and Stat-3 and Sox-5, in TMJ tissue samples from human fetuses aged between the 12th and 20th weeks of intrauterine life.
UNASSIGNED: We fixed and processed TMJ tissue samples from human fetuses, histological sections and immunohistochemical procedures were carried out.
UNASSIGNED: TMJ histological studies examination did not reveal any difference in the tissue organization between the samples in the studied periods. Immunohistochemical analysis demonstrated that Oct-4 and Sox-2 lack their expression in TMJ. In contrast, Nanog was expressed in nucleous of proliferative layer of mandibular condyle, Stat-3 was expressed in nuclear cells of articular disc, Stat-3 and Sox-5 showed positive nuclear and cytoplasmic immunostaining in codrocyte layers and in ossification areas.
UNASSIGNED: Nanog acts in maintanence of pluripotency, Stat-3 in articular disc acts as a transcriptional factor. Stat-3 and Sox-2 act in chondrocyte and osteoblast diferentiation. Distribution of the cells, which express Nanog, Stat-3, and Sox-5 in TMJ tissue during fetal development, can help further understand its physiology, pathology, and repairing capacities.
UNASSIGNED: We fixed and processed TMJ tissue samples from human fetuses, histological sections and immunohistochemical procedures were carried out.
UNASSIGNED: TMJ histological studies examination did not reveal any difference in the tissue organization between the samples in the studied periods. Immunohistochemical analysis demonstrated that Oct-4 and Sox-2 lack their expression in TMJ. In contrast, Nanog was expressed in nucleous of proliferative layer of mandibular condyle, Stat-3 was expressed in nuclear cells of articular disc, Stat-3 and Sox-5 showed positive nuclear and cytoplasmic immunostaining in codrocyte layers and in ossification areas.
UNASSIGNED: Nanog acts in maintanence of pluripotency, Stat-3 in articular disc acts as a transcriptional factor. Stat-3 and Sox-2 act in chondrocyte and osteoblast diferentiation. Distribution of the cells, which express Nanog, Stat-3, and Sox-5 in TMJ tissue during fetal development, can help further understand its physiology, pathology, and repairing capacities.
摘要：
■颞下颌关节（TMJ）是一种滑膜关节，可以进行生命必不可少的复杂运动。它把颚骨和头骨连接起来,作为一个滑动铰链。此外，多能干细胞是发育中生物体的前体和组织特异性细胞的来源，然而,它们在发育中的胎儿组织中的生物分布研究薄弱。我们研究的目的是分析Nanog的免疫组织化学表达，Oct-4，Sox-2和Stat-3和Sox-5，来自子宫内寿命第12至20周的人类胎儿的TMJ组织样本。
■我们固定并处理了来自人类胎儿的TMJ组织样本，进行组织切片和免疫组织化学程序。
■TMJ组织学研究检查未揭示在研究期间样品之间的组织组织的任何差异。免疫组织化学分析表明Oct-4和Sox-2在TMJ中缺乏表达。相比之下,Nanog在下颌髁突增殖层的核中表达，Stat-3在关节盘的核细胞中表达，Stat-3和Sox-5在软骨层和骨化区域显示出阳性的核和细胞质免疫染色。
■Nanog在多能性的维持中发挥作用，关节盘中的stat-3充当转录因子。Stat-3和Sox-2在软骨细胞和成骨细胞分化中起作用。细胞的分布,表示Nanog，胎儿发育过程中TMJ组织中的Stat-3和Sox-5，可以帮助进一步了解它的生理学，病理学,和修复能力。
■我们固定并处理了来自人类胎儿的TMJ组织样本，进行组织切片和免疫组织化学程序。
■TMJ组织学研究检查未揭示在研究期间样品之间的组织组织的任何差异。免疫组织化学分析表明Oct-4和Sox-2在TMJ中缺乏表达。相比之下,Nanog在下颌髁突增殖层的核中表达，Stat-3在关节盘的核细胞中表达，Stat-3和Sox-5在软骨层和骨化区域显示出阳性的核和细胞质免疫染色。
■Nanog在多能性的维持中发挥作用，关节盘中的stat-3充当转录因子。Stat-3和Sox-2在软骨细胞和成骨细胞分化中起作用。细胞的分布,表示Nanog，胎儿发育过程中TMJ组织中的Stat-3和Sox-5，可以帮助进一步了解它的生理学，病理学,和修复能力。
