PDF(Pubmed)
Abstract：
Harness® is a commercial herbicide that contains acetochlor at a concentration of 84% as an active ingredient. Ubiquitous, persistent, and substantial uses of Harness® in agricultural processes have resulted in the pollution of nearby water sources, posing a threat to various aquatic biotas, including fish. The effects of Harness® toxicity on fish health are little known. So, this study aimed to describe the impact of herbicide Harness® on the oxidative stress and reproductive and thyroid performance of male and female tilapia (Oreochromis niloticus) and also investigate the prospective role of the natural antioxidant lycopene supplementation in dismissing the adverse properties of Harness®. Antioxidant enzyme (catalase, superoxide dismutase, and total antioxidant capacity) and hormone measurements (T, E2, T3, and T4) were carried out, and gonadal and thyroid follicle histological sections were examined as a method to investigate the effects of Harness® toxicity on fish. Male and female tilapia were exposed to 10 μmol/L and 100 μmol/L of Harness® and treated with 10 mg lycopene/kg for 15 days of exposure. Our results demonstrated that the antioxidant enzyme activity was altered by Harness exposure and serum T for both males and females dropped; also, female E2 levels decreased, but male E2 increased. Exposure to higher dose of Harness® induced elevation in both T3 and T4 levels, although the low exposure dose stimulated T4 levels. Harness® exposure prompted histological variations and degenerative changes in testicular, ovarian, and thyroid follicle tissues. Lycopene supplement administration diminished oxidative stress induced by Harness®, alleviating its endocrine disparaging effects by neutralizing T3, T4, T, and E2 and ameliorating the histological structure of gonadal and thyroid tissues. In conclusion, lycopene supplementation was preformed to normalize the alterations and oxidative damage caused by Harness® in Nile tilapia, suggesting that lycopene-supplemented diet functioned as potent antioxidants and had the ability to alleviate oxidative stress and thyroid and reproductive toxicity caused by herbicide Harness®. Moreover, it is crucial to take appropriate care when consuming herbicides to defend the aquatic environment.
摘要：
Harness®是一种商业除草剂,其含有浓度为84%的乙草胺作为活性成分。无处不在，持久性,在农业过程中大量使用Harness®导致了附近水源的污染，对各种水生生物构成威胁，包括鱼。Harness®毒性对鱼类健康的影响鲜为人知。所以,这项研究旨在描述除草剂Harness®对雄性和雌性罗非鱼（Oreochromisniloticus）的氧化应激以及生殖和甲状腺性能的影响，并研究补充天然抗氧化剂番茄红素在消除Harness®不利特性方面的预期作用。抗氧化酶（过氧化氢酶，超氧化物歧化酶,和总抗氧化能力)和激素测量(T，E2，T3和T4）进行，检查了性腺和甲状腺滤泡组织切片，以研究Harness®毒性对鱼类的影响。将雄性和雌性罗非鱼暴露于10μmol/L和100μmol/L的Harness®,并用10mg番茄红素/kg处理15天暴露。我们的结果表明，抗氧化剂酶活性因线束暴露而改变，男性和女性的血清T下降；同时，女性E2水平下降，但男性E2增加。暴露于较高剂量的Harness®导致T3和T4水平升高，虽然低暴露剂量刺激T4水平。Harness®暴露促使睾丸组织学变化和退行性变化，卵巢,和甲状腺滤泡组织。番茄红素补充剂的给药减少了由Harness®引起的氧化应激，通过中和T3、T4、T、和E2，改善性腺和甲状腺组织的组织学结构。总之,补充番茄红素是为了使由Harness®在尼罗罗非鱼中引起的改变和氧化损伤正常化，这表明补充番茄红素的饮食可以作为有效的抗氧化剂，并且能够减轻除草剂Harness®引起的氧化应激以及甲状腺和生殖毒性。此外，在食用除草剂以保护水生环境时，采取适当的措施至关重要。
