PDF(Pubmed)
Abstract：
Hydrolytic reactions taking place at the surface of a silicon nitride (Si3N4) bioceramic were found to induce instantaneous inactivation of Human herpesvirus 1 (HHV-1, also known as Herpes simplex virus 1 or HSV-1). Si3N4 is a non-oxide ceramic compound with strong antibacterial and antiviral properties that has been proven safe for human cells. HSV-1 is a double-stranded DNA virus that infects a variety of host tissues through a lytic and latent cycle. Real-time reverse transcription (RT)-polymerase chain reaction (PCR) tests of HSV-1 DNA after instantaneous contact with Si3N4 showed that ammonia and its nitrogen radical byproducts, produced upon Si3N4 hydrolysis, directly reacted with viral proteins and fragmented the virus DNA, irreversibly damaging its structure. A comparison carried out upon testing HSV-1 against ZrO2 particles under identical experimental conditions showed a significantly weaker (but not null) antiviral effect, which was attributed to oxygen radical influence. The results of this study extend the effectiveness of Si3N4\'s antiviral properties beyond their previously proven efficacy against a large variety of single-stranded enveloped and non-enveloped RNA viruses. Possible applications include the development of antiviral creams or gels and oral rinses to exploit an extremely efficient, localized, and instantaneous viral reduction by means of a safe and more effective alternative to conventional antiviral creams. Upon incorporating a minor fraction of micrometric Si3N4 particles into polymeric matrices, antiherpetic devices could be fabricated, which would effectively impede viral reactivation and enable high local effectiveness for extended periods of time.
摘要：
发现在氮化硅（Si3N4）生物陶瓷表面发生的水解反应可诱导人疱疹病毒1（HHV-1，也称为单纯疱疹病毒1或HSV-1）的瞬时失活。Si3N4是一种非氧化物陶瓷化合物，具有很强的抗菌和抗病毒性能，已被证明对人类细胞是安全的。HSV-1是通过裂解和潜伏循环感染多种宿主组织的双链DNA病毒。与Si3N4瞬时接触后的HSV-1DNA的实时逆转录（RT）-聚合酶链反应（PCR）测试表明，氨及其氮自由基副产物，Si3N4水解产生，直接与病毒蛋白反应并片段化病毒DNA，不可逆转地破坏其结构。在相同的实验条件下测试HSV-1对ZrO2颗粒进行的比较显示出明显较弱（但不无效）的抗病毒作用。这归因于氧自由基的影响。这项研究的结果扩展了Si3N4的抗病毒特性的有效性，超出了其先前证明的对各种单链包膜和无包膜RNA病毒的功效。可能的应用包括开发抗病毒乳膏或凝胶和口腔冲洗剂，以利用极其有效的，局部化,和瞬时病毒减少通过一个安全和更有效的替代传统的抗病毒药膏。在将一小部分微米Si3N4颗粒掺入聚合物基质中时，可以制造抗疱疹装置，这将有效地阻止病毒的再激活，并在延长的时间内实现高的局部有效性。
