METHODS: We evaluated the differences between GBMCs and astrocytes in terms of the abovementioned parameters and assessed them with the aim of studying human GBMCs (U-87 MG) and astrocytes (SVG p12). The cells were exposed to different doses of the JNK inhibitor, SP600125, for 24, 48, and 72 hours. HIF-1α, BMP4, and F-actin expressions were evaluated using immunofluorescence image analysis.
RESULTS: The half-maximal inhibitory concentration value for SP600125 was determined to be 10 μM at 24 hours of exposure. After SP600125 administration, elevated levels of HIF-1α and BMP4 were detected in GBMCs and astrocytes. F-actin level only increased in GBMCs after SP600125 administration.
CONCLUSIONS: JNKs are important for cell proliferation, differentiation, survival, and death; thus, research on JNKs has become important for the treatment of many human diseases, especially brain tumors, Parkinson\'s disease, and Alzheimer\'s disease. The results of this study involving immunofluorescence techniques should be investigated and supported by studies that involve comprehensive molecular techniques.
方法:我们评估了GBMC和星形胶质细胞在上述参数方面的差异,并评估了它们,目的是研究人GBMC(U-87MG)和星形胶质细胞(SVGp12)。细胞暴露于不同剂量的JNK抑制剂,SP600125,持续24、48和72小时。HIF-1α,使用免疫荧光图像分析评估BMP4和F-肌动蛋白表达。
结果:SP600125的半最大抑制浓度值在暴露24小时时测定为10μM。SP600125管理后,在GBMC和星形胶质细胞中检测到HIF-1α和BMP4水平升高。在SP600125施用后,F-肌动蛋白水平仅在GBMC中增加。
结论:JNK对细胞增殖很重要,分化,生存,和死亡;因此,对JNKs的研究已经成为治疗许多人类疾病的重要手段,尤其是脑肿瘤,帕金森病,和老年痴呆症。这项涉及免疫荧光技术的研究结果应得到涉及综合分子技术的研究的调查和支持。