PDF(Pubmed)
Abstract：
UNASSIGNED: As prostaglandin E2 (PGE2) has important roles in physiological and inflammatory functions, a double-blind randomized controlled crossover study to investigate the potential of nasturtium (Tropaeolum majus) for modulating PGE2 was conducted, aiming at clarifying the role of benzyl isothiocyanate (BITC). As secondary parameters leukotriene 4 (LTB4), and cytokine release (tumor necrosis factor alpha, TNF-α; interleukins IL-1β, IL-10, and IL-12) were quantified.
UNASSIGNED: Thirty-four healthy female participants consumed 1.5 g nasturtium containing BITC, (verum) or no BITC (control) twice a day for 2 weeks each. Nasturtium intervention resulted in an increase in mean PGE2 levels in serum samples (verum: 1.76-fold, p ≤ 0.05; control: 1.78-fold, p ≤ 0.01), and ex vivo stimulated peripheral blood mononuclear cells (PBMC) (verum: 1.71-fold, p ≤ 0.01; control: 1.43-fold). Using a pre-to-post responder analysis approach, 18 of 34 subjects showed a > 25% PGE2 increase in serum, while it was >25% decreased for 9 subjects (stimulated PBMC: 14 and 8 of 28, respectively). Under the selected conditions, the BITC content of nasturtium did not affect the observed changes in PGE2. Verum intervention also increased mean LTB4 serum level (1.24-fold, p ≤ 0.01), but not in LPS stimulated PBMC, and significantly increased TNF-α release in stimulated PBMC after 3 h (verum: 1.65-fold, p = 0.0032; control: 1.22-fold, p = 0.7818). No change was seen in the anti-inflammatory cytokine IL-10, or the pro-inflammatory cytokines IL-1β, and IL-12.
UNASSIGNED: In contrast to the previously reported in vitro results, on average, LPS activated PBMC and serum from both groups showed increased PGE2 levels. Further analyses suggest that PGE2 release after intervention could possibly depend on the baseline PGE2 level. Identification of phenotypes that respond differently to the nasturtium intervention could be useful to establish personalized approaches for dosing phytopharmaceuticals medicines.
UNASSIGNED: Thirty-four healthy female participants consumed 1.5 g nasturtium containing BITC, (verum) or no BITC (control) twice a day for 2 weeks each. Nasturtium intervention resulted in an increase in mean PGE2 levels in serum samples (verum: 1.76-fold, p ≤ 0.05; control: 1.78-fold, p ≤ 0.01), and ex vivo stimulated peripheral blood mononuclear cells (PBMC) (verum: 1.71-fold, p ≤ 0.01; control: 1.43-fold). Using a pre-to-post responder analysis approach, 18 of 34 subjects showed a > 25% PGE2 increase in serum, while it was >25% decreased for 9 subjects (stimulated PBMC: 14 and 8 of 28, respectively). Under the selected conditions, the BITC content of nasturtium did not affect the observed changes in PGE2. Verum intervention also increased mean LTB4 serum level (1.24-fold, p ≤ 0.01), but not in LPS stimulated PBMC, and significantly increased TNF-α release in stimulated PBMC after 3 h (verum: 1.65-fold, p = 0.0032; control: 1.22-fold, p = 0.7818). No change was seen in the anti-inflammatory cytokine IL-10, or the pro-inflammatory cytokines IL-1β, and IL-12.
UNASSIGNED: In contrast to the previously reported in vitro results, on average, LPS activated PBMC and serum from both groups showed increased PGE2 levels. Further analyses suggest that PGE2 release after intervention could possibly depend on the baseline PGE2 level. Identification of phenotypes that respond differently to the nasturtium intervention could be useful to establish personalized approaches for dosing phytopharmaceuticals medicines.
摘要：
■由于前列腺素E2（PGE2）在生理和炎症功能中具有重要作用，进行了一项双盲随机对照交叉研究，以研究金莲花（Tropaeolummajus）调节PGE2的潜力，旨在阐明异硫氰酸苄酯（BITC）的作用。作为次要参数白三烯4(LTB4)，和细胞因子释放(肿瘤坏死因子α，TNF-α;白细胞介素类IL-1β,对IL-10和IL-12)进行定量。
■34名健康的女性参与者食用了1.5克含有BITC的金莲花，（verum）或无BITC（对照）每天两次，每次2周。Sturum干预导致血清样本中平均PGE2水平增加（verum：1.76倍，p≤0.05；对照：1.78倍，p≤0.01),和离体刺激的外周血单核细胞（PBMC）（verum：1.71倍，p≤0.01；对照：1.43倍）。使用前到后的响应者分析方法，34名受试者中有18名显示血清PGE2增加>25%，而9名受试者的下降>25%(刺激的PBMC:28人中的14人和8人)。在选定的条件下，金莲花的BITC含量不影响观察到的PGE2变化。Verum干预也增加了平均LTB4血清水平(1.24倍，p≤0.01),但不在LPS刺激的PBMC中，3小时后，刺激的PBMC中TNF-α的释放显着增加（verum：1.65倍，p=0.0032；对照：1.22倍，p=0.7818）。抗炎细胞因子IL-10或促炎细胞因子IL-1β未见变化，IL-12
■与先前报道的体外结果相反，平均而言,来自两组的LPS激活的PBMC和血清显示升高的PGE2水平。进一步的分析表明，干预后PGE2的释放可能取决于基线PGE2水平。鉴定对金莲花干预有不同反应的表型可用于建立给药植物药物的个性化方法。
■34名健康的女性参与者食用了1.5克含有BITC的金莲花，（verum）或无BITC（对照）每天两次，每次2周。Sturum干预导致血清样本中平均PGE2水平增加（verum：1.76倍，p≤0.05；对照：1.78倍，p≤0.01),和离体刺激的外周血单核细胞（PBMC）（verum：1.71倍，p≤0.01；对照：1.43倍）。使用前到后的响应者分析方法，34名受试者中有18名显示血清PGE2增加>25%，而9名受试者的下降>25%(刺激的PBMC:28人中的14人和8人)。在选定的条件下，金莲花的BITC含量不影响观察到的PGE2变化。Verum干预也增加了平均LTB4血清水平(1.24倍，p≤0.01),但不在LPS刺激的PBMC中，3小时后，刺激的PBMC中TNF-α的释放显着增加（verum：1.65倍，p=0.0032；对照：1.22倍，p=0.7818）。抗炎细胞因子IL-10或促炎细胞因子IL-1β未见变化，IL-12
■与先前报道的体外结果相反，平均而言,来自两组的LPS激活的PBMC和血清显示升高的PGE2水平。进一步的分析表明，干预后PGE2的释放可能取决于基线PGE2水平。鉴定对金莲花干预有不同反应的表型可用于建立给药植物药物的个性化方法。
