PDF(Pubmed)
Abstract：
OBJECTIVE: The protective effect of aqueous and methanolic extracts of corn silk on reproductive disorders induced by nicotine was investigated in the present study.
METHODS: In this experimental study, 30 male NMRI mice (25-30gr) were divided into 5 groups: controls, sham, nicotine 2.5mg/kg, nicotine+aqueous extract of corn silk 400mg/kg, and nicotine+methanolic extract of corn silk 400mg/kg for 34 days. One day after the last nicotine and extracts administration, the serum samples were collected through cardiac puncture for hormonal measurements, and the testis and tail of the epididymis were isolated for the testis antioxidant, morphology, histopathology assessments, and sperm count.
RESULTS: Luteinizing hormone (LH) and malondialdehyde (MDA) increased in the nicotine group. Testosterone, sperm count, and glutathione (GSH) decreased when compared to the control group. Both aqueous and methanolic extracts of corn silk led to the improvement of mentioned changes; Except for GSH, because only treatment with methanolic extract could lead to its increase (p<0.05). Nicotine decreased the thickness of the epithelium of seminiferous tubules and the separation between them, and the administration of corn silk extracts improved that.
CONCLUSIONS: Nicotine consumption increased oxidative stress, LH levels, and decreased testosterone and sperm count, which indicate the induction of primary hypogonadism in animals. Moreover, the use of corn silk extracts has recovered the amounts of sex hormones and sperm count to normal conditions by reducing lipid peroxidation.
METHODS: In this experimental study, 30 male NMRI mice (25-30gr) were divided into 5 groups: controls, sham, nicotine 2.5mg/kg, nicotine+aqueous extract of corn silk 400mg/kg, and nicotine+methanolic extract of corn silk 400mg/kg for 34 days. One day after the last nicotine and extracts administration, the serum samples were collected through cardiac puncture for hormonal measurements, and the testis and tail of the epididymis were isolated for the testis antioxidant, morphology, histopathology assessments, and sperm count.
RESULTS: Luteinizing hormone (LH) and malondialdehyde (MDA) increased in the nicotine group. Testosterone, sperm count, and glutathione (GSH) decreased when compared to the control group. Both aqueous and methanolic extracts of corn silk led to the improvement of mentioned changes; Except for GSH, because only treatment with methanolic extract could lead to its increase (p<0.05). Nicotine decreased the thickness of the epithelium of seminiferous tubules and the separation between them, and the administration of corn silk extracts improved that.
CONCLUSIONS: Nicotine consumption increased oxidative stress, LH levels, and decreased testosterone and sperm count, which indicate the induction of primary hypogonadism in animals. Moreover, the use of corn silk extracts has recovered the amounts of sex hormones and sperm count to normal conditions by reducing lipid peroxidation.
摘要：
目的：研究玉米丝水提物和甲醇提物对尼古丁所致生殖障碍的保护作用。
方法：在本实验研究中，将30只雄性NMRI小鼠（25-30gr）分为5组：对照组，sham,尼古丁2.5mg/kg，尼古丁+玉米丝水提取物400mg/kg，烟碱+玉米丝甲醇提取物400mg/kg,连续34天。最后一次服用尼古丁和提取物后的一天，通过心脏穿刺收集血清样本进行激素测量，睾丸和附睾的尾巴被分离为睾丸抗氧化剂，形态学,组织病理学评估，和精子计数。
结果：尼古丁组黄体生成素（LH）和丙二醛（MDA）增加。睾酮,精子计数,与对照组相比，谷胱甘肽（GSH）降低。玉米丝的水溶液和甲醇提取物均导致上述变化的改善；除GSH外，因为仅用甲醇提取物处理可导致其增加（p<0.05）。尼古丁降低了生精小管上皮的厚度和它们之间的分离，玉米丝提取物的管理改善了这一点。
结论：尼古丁消耗增加了氧化应激，LH水平,睾丸激素和精子数量减少，这表明动物原发性性腺功能减退症的诱导。此外，玉米丝提取物的使用通过减少脂质过氧化，使性激素和精子数量恢复到正常状态。
方法：在本实验研究中，将30只雄性NMRI小鼠（25-30gr）分为5组：对照组，sham,尼古丁2.5mg/kg，尼古丁+玉米丝水提取物400mg/kg，烟碱+玉米丝甲醇提取物400mg/kg,连续34天。最后一次服用尼古丁和提取物后的一天，通过心脏穿刺收集血清样本进行激素测量，睾丸和附睾的尾巴被分离为睾丸抗氧化剂，形态学,组织病理学评估，和精子计数。
结果：尼古丁组黄体生成素（LH）和丙二醛（MDA）增加。睾酮,精子计数,与对照组相比，谷胱甘肽（GSH）降低。玉米丝的水溶液和甲醇提取物均导致上述变化的改善；除GSH外，因为仅用甲醇提取物处理可导致其增加（p<0.05）。尼古丁降低了生精小管上皮的厚度和它们之间的分离，玉米丝提取物的管理改善了这一点。
结论：尼古丁消耗增加了氧化应激，LH水平,睾丸激素和精子数量减少，这表明动物原发性性腺功能减退症的诱导。此外，玉米丝提取物的使用通过减少脂质过氧化，使性激素和精子数量恢复到正常状态。
