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Abstract：
This study aims to explore the relationship between macrosomia and amino acids in maternal and cord sera.
METHODS: In the case-control study, 78 pairs of mothers and newborns were recruited from December 2016 to November 2019. Participants were divided into the macrosomia group (BW ≥ 4000 g, n = 39) and the control group (BW between 2500 g and 3999 g, n = 39) according to the birth weight (BW) of newborns. Maternal vein blood samples were collected before delivery and cord vein blood samples were collected after birth. The levels of amino acids in maternal and cord sera were measured by liquid chromatography and mass spectrometry (LC-MS/MS) in the year 2021. The difference in amino acid levels in maternal and cord sera between the two groups was compared, and the contribution of each amino acid to the difference between the two groups was analyzed. Unconditional logistic regression analysis was used to test the relationship between macrosomia and amino acids.
RESULTS: In maternal serum during the antepartum, the levels of asparagine, glutamine, methionine, alanine, and threonine in the macrosomia group were higher but arginine was lower than that in the control group (p < 0.05). In cord serum, the levels of lysine, histidine, phenylalanine, arginine, tryptophan, valine, isoleucine, glutamate, tyrosine, and total essential amino acid (EAA) in the macrosomia group were lower while glutamine was higher than that in the control group (p < 0.05). The ratios of EAA, valine, threonine, methionine, tryptophan, and alanine in maternal serum to those in cord serum were higher, while the ratio of glutamine was lower in the macrosomia group (p < 0.05). Arginine and threonine in maternal serum and glutamate, glutamine, and histidine in cord serum were associated with macrosomia (p < 0.05).
CONCLUSIONS: Most of the amino acid levels in the maternal sera of the macrosomia group are higher than those in the control group, while most of the amino acids\' levels in the cord sera of the macrosomia group are lower than those in the control group. The ratios of some amino acids in maternal serum to those in cord serum were different between the two groups. Arginine and threonine in maternal serum and glutamate, glutamine, and histidine in cord serum are closely related to macrosomia.
METHODS: In the case-control study, 78 pairs of mothers and newborns were recruited from December 2016 to November 2019. Participants were divided into the macrosomia group (BW ≥ 4000 g, n = 39) and the control group (BW between 2500 g and 3999 g, n = 39) according to the birth weight (BW) of newborns. Maternal vein blood samples were collected before delivery and cord vein blood samples were collected after birth. The levels of amino acids in maternal and cord sera were measured by liquid chromatography and mass spectrometry (LC-MS/MS) in the year 2021. The difference in amino acid levels in maternal and cord sera between the two groups was compared, and the contribution of each amino acid to the difference between the two groups was analyzed. Unconditional logistic regression analysis was used to test the relationship between macrosomia and amino acids.
RESULTS: In maternal serum during the antepartum, the levels of asparagine, glutamine, methionine, alanine, and threonine in the macrosomia group were higher but arginine was lower than that in the control group (p < 0.05). In cord serum, the levels of lysine, histidine, phenylalanine, arginine, tryptophan, valine, isoleucine, glutamate, tyrosine, and total essential amino acid (EAA) in the macrosomia group were lower while glutamine was higher than that in the control group (p < 0.05). The ratios of EAA, valine, threonine, methionine, tryptophan, and alanine in maternal serum to those in cord serum were higher, while the ratio of glutamine was lower in the macrosomia group (p < 0.05). Arginine and threonine in maternal serum and glutamate, glutamine, and histidine in cord serum were associated with macrosomia (p < 0.05).
CONCLUSIONS: Most of the amino acid levels in the maternal sera of the macrosomia group are higher than those in the control group, while most of the amino acids\' levels in the cord sera of the macrosomia group are lower than those in the control group. The ratios of some amino acids in maternal serum to those in cord serum were different between the two groups. Arginine and threonine in maternal serum and glutamate, glutamine, and histidine in cord serum are closely related to macrosomia.
摘要：
本研究旨在探讨巨大儿与母体和脐带血清中氨基酸的关系。
方法：在病例对照研究中，从2016年12月至2019年11月，招募了78对母亲和新生儿。参与者被分为巨大儿组(体重≥4000克，n=39）和对照组（BW在2500g至3999g之间，n=39）根据新生儿的出生体重（BW）。分娩前收集母体静脉血样，出生后收集脐静脉血样。2021年通过液相色谱和质谱（LC-MS/MS）测量了母体和脐带血中的氨基酸水平。比较两组孕妇血清和脐带血中氨基酸水平的差异,分析各氨基酸对两组间差异的贡献。非条件Logistic回归分析用于检验巨大儿与氨基酸之间的关系。
结果：在产前母体血清中，天冬酰胺的水平，谷氨酰胺,蛋氨酸,丙氨酸,巨大儿组苏氨酸高于对照组，精氨酸低于对照组（p<0.05）。在脐带血清中，赖氨酸的水平，组氨酸,苯丙氨酸,精氨酸色氨酸,缬氨酸,异亮氨酸,谷氨酸,酪氨酸,巨大儿组的总必需氨基酸（EAA）低于对照组，而谷氨酰胺高于对照组（p<0.05）。EAA的比率，缬氨酸,苏氨酸,蛋氨酸,色氨酸,母体血清中的丙氨酸高于脐带血清中的丙氨酸，而巨大儿组中谷氨酰胺的比例较低（p<0.05）。母体血清和谷氨酸中的精氨酸和苏氨酸，谷氨酰胺,脐带血组氨酸与巨大儿相关(p<0.05)。
结论:巨大儿组母体血清中的氨基酸水平大多高于对照组,而巨大儿组脐带血清中的大多数氨基酸水平低于对照组。两组孕妇血清中某些氨基酸与脐带血清中某些氨基酸的比率不同。母体血清和谷氨酸中的精氨酸和苏氨酸，谷氨酰胺,脐带血中组氨酸与巨大儿密切相关。
方法：在病例对照研究中，从2016年12月至2019年11月，招募了78对母亲和新生儿。参与者被分为巨大儿组(体重≥4000克，n=39）和对照组（BW在2500g至3999g之间，n=39）根据新生儿的出生体重（BW）。分娩前收集母体静脉血样，出生后收集脐静脉血样。2021年通过液相色谱和质谱（LC-MS/MS）测量了母体和脐带血中的氨基酸水平。比较两组孕妇血清和脐带血中氨基酸水平的差异,分析各氨基酸对两组间差异的贡献。非条件Logistic回归分析用于检验巨大儿与氨基酸之间的关系。
结果：在产前母体血清中，天冬酰胺的水平，谷氨酰胺,蛋氨酸,丙氨酸,巨大儿组苏氨酸高于对照组，精氨酸低于对照组（p<0.05）。在脐带血清中，赖氨酸的水平，组氨酸,苯丙氨酸,精氨酸色氨酸,缬氨酸,异亮氨酸,谷氨酸,酪氨酸,巨大儿组的总必需氨基酸（EAA）低于对照组，而谷氨酰胺高于对照组（p<0.05）。EAA的比率，缬氨酸,苏氨酸,蛋氨酸,色氨酸,母体血清中的丙氨酸高于脐带血清中的丙氨酸，而巨大儿组中谷氨酰胺的比例较低（p<0.05）。母体血清和谷氨酸中的精氨酸和苏氨酸，谷氨酰胺,脐带血组氨酸与巨大儿相关(p<0.05)。
结论:巨大儿组母体血清中的氨基酸水平大多高于对照组,而巨大儿组脐带血清中的大多数氨基酸水平低于对照组。两组孕妇血清中某些氨基酸与脐带血清中某些氨基酸的比率不同。母体血清和谷氨酸中的精氨酸和苏氨酸，谷氨酰胺,脐带血中组氨酸与巨大儿密切相关。
