PDF(Pubmed)
Abstract：
Recently, consumer preferences for bilberries have increased markedly. This fact is probably related to their natural constituents, such as phenolic compounds including anthocyanins and tannins, as well as the vitamins and minerals they contain. Phenolic compounds are known for their numerous beneficial effects on human health. Moreover, bilberry fruits have been shown to inhibit the activity of carbohydrate hydrolyzing enzymes, which can significantly decrease the postprandial increase in blood glucose levels. Thus, the aim of the present study is to investigate the inhibitory effect of Vaccinium myrtillus L. extracts on key enzyme α-amylase, linked to type 2 diabetes. No data have been published on the inhibitory properties of Vaccinium myrtillus L. fruits growing wild in Bulgaria against carbohydrate enzymes. Bilberry extracts were analyzed for total polyphenols, total anthocyanin content, antioxidant activity and their inhibitory properties against α-amylase. The contents of flavonols, anthocyanins and stilbenes were determined by HPLC analysis. The identified flavonols in the analyzed bilberry extracts were mainly represented by quercetin derivatives as rutinoside. The predominant anthocyanins for both aqueous and organic solvents were delphinidin-3-galactoside and malvidin-3-glucoside. The results revealed that bilberry extracts are effective inhibitors of α-amylase, with IC50 values from 20.8 to 194.8 μg GAE/mL. All the samples proved to have antioxidant activity measured by three different in vitro assays (FRAP, CUPRAC and DPPH). The inhibitory properties of V. myrtillus L. extracts may provide a new direction in the development and research of new pharmaceuticals for the suppression of postprandial hyperglycemia in diabetic patients.
摘要：
最近,消费者对越橘的偏好明显增加。这个事实可能与它们的天然成分有关，如酚类化合物，包括花青素和单宁，以及它们含有的维生素和矿物质。酚类化合物因其对人类健康的许多有益作用而为人所知。此外，越橘果实已被证明能抑制碳水化合物水解酶的活性，可以显着降低餐后血糖水平的升高。因此，本研究的目的是研究麦片提取物对关键酶α-淀粉酶的抑制作用,与2型糖尿病有关。尚未发表有关在保加利亚野生生长的VacciniummyrtillusL.果实对碳水化合物酶的抑制特性的数据。分析了越橘提取物的总多酚，总花色苷含量，抗氧化活性及其对α-淀粉酶的抑制特性。黄酮醇的含量，通过HPLC分析测定花色苷和二苯乙烯。所分析的越橘提取物中鉴定出的黄酮醇主要以槲皮素衍生物为代表。水性溶剂和有机溶剂中的主要花色苷是delphinidin-3-半乳糖苷和malvidin-3-葡萄糖苷。结果表明,越橘提取物是α-淀粉酶的有效抑制剂,IC50值为20.8至194.8μgGAE/mL。通过三种不同的体外测定法（FRAP，CUPRAC和DPPH)。苦参提取物的抑制特性可能为抑制糖尿病患者餐后高血糖新药的开发和研究提供新的方向。
