PDF(Pubmed)
Abstract：
The antimicrobial properties of baicalin against H. pylori and several probiotic cultures were evaluated. Baicalin was isolated from a dry plant extract obtained by extraction with water at 70 °C. For isolation, extraction was carried out with n-butanol and purification on a chromatographic column. The antimicrobial potential was assessed by evaluating changes in the optical density of the bacterial suspension during cultivation; additionally, the disk diffusion method was used. During the study, the baicalin concentrations (0.25, 0.5, and 1 mg/mL) and the pH of the medium in the range of 1.5-8.0 were tested. The test objects were: suspensions of H. pylori, Lactobacillus casei, L. brevis, Bifidobacterium longum, and B. teenis. It was found that the greater the concentration of the substance in the solution, the greater the delay in the growth of the strain zone. Thus, the highest antimicrobial activity against H. pylori was observed at pH 1.5-2.0 and a baicalin concentration of 1.00 mg/mL. In relation to probiotic strains, a stimulating effect of baicalin (1.00 mg/mL) on the growth of L. casei biomass at pH 1.5-2.0 was observed. The results open up the prospects for the use of baicalin and probiotics for the treatment of diseases caused by H. pylori.
摘要：
评价了黄芩苷对幽门螺杆菌和几种益生菌培养物的抗菌性能。从通过在70°C下用水提取获得的干燥植物提取物中分离黄芩苷。为了隔离，用正丁醇进行萃取并在色谱柱上纯化。通过评估培养过程中细菌悬浮液的光密度变化来评估抗微生物潜力；此外，采用圆盘扩散法。在研究期间,测试了黄芩苷的浓度（0.25、0.5和1mg/mL）和培养基的pH在1.5-8.0范围内。试验对象为：幽门螺杆菌悬液，干酪乳杆菌,L.brevis,长双歧杆菌，还有B.teenis.发现溶液中物质的浓度越大，应变区生长的延迟越大。因此，在pH1.5-2.0和黄芩苷浓度为1.00mg/mL时,观察到对幽门螺杆菌的抗菌活性最高.关于益生菌菌株，观察到黄芩苷（1.00mg/mL）对pH1.5-2.0的干酪乳杆菌生物量生长的刺激作用。该成果为黄芩苷和益生菌用于医治H.pylori惹起的疾病开辟了远景。
