PDF(Pubmed)
Abstract：
BACKGROUND: Angiostrongylus cantonensis (rat lungworm) is the main pathogen responsible for eosinophilic meningitis in humans. One of its intermediate snail hosts, Achatina fulica, was already present in many countries around the world before it appeared in the West Indies in the late 1980s. In the French territories in the Caribbean and northern South America, the first cases of human neuroangiostrongyliasis were reported in Martinique, Guadeloupe and French Guiana in 2002, 2013 and 2017, respectively. In order to better characterize angiostrongyliasis in Guadeloupe, particularly its geographical origin and route of introduction, we undertook molecular characterization of adult worms of Angiostrongylus cantonensis and its intermediate host Achatina fulica.
METHODS: Genomic DNA of adult Angiostrongylus cantonensis and Achatina fulica was extracted and amplified by polymerase chain reaction (PCR) targeting the mitochondrial genes cytochrome B and C for A. cantonensis and 16S ribosomal RNA for A. fulica. The PCR products were sequenced and studied by phylogenetic analysis.
RESULTS: Cytochrome B and cytochrome C molecular markers indicate a monophyletic lineage of A. cantonensis adult worms in Guadeloupe. Two sequences of A. fulica were identified.
CONCLUSIONS: These results confirm the recent introduction of both Angiostrongylus cantonensis and Achatina fulica into Guadeloupe. Achatina fulica in Guadeloupe shares a common origin with those in Barbados and New Caledonia, while Angiostrongylus cantonensis in Guadeloupe shares a common origin with those in Brazil, Hawaii and Japan.
METHODS: Genomic DNA of adult Angiostrongylus cantonensis and Achatina fulica was extracted and amplified by polymerase chain reaction (PCR) targeting the mitochondrial genes cytochrome B and C for A. cantonensis and 16S ribosomal RNA for A. fulica. The PCR products were sequenced and studied by phylogenetic analysis.
RESULTS: Cytochrome B and cytochrome C molecular markers indicate a monophyletic lineage of A. cantonensis adult worms in Guadeloupe. Two sequences of A. fulica were identified.
CONCLUSIONS: These results confirm the recent introduction of both Angiostrongylus cantonensis and Achatina fulica into Guadeloupe. Achatina fulica in Guadeloupe shares a common origin with those in Barbados and New Caledonia, while Angiostrongylus cantonensis in Guadeloupe shares a common origin with those in Brazil, Hawaii and Japan.
摘要：
背景：广州管圆线虫（大鼠肺虫）是人类嗜酸性粒细胞性脑膜炎的主要病原体。它的中间蜗牛宿主之一，AchatinaFulica,在1980年代后期出现在西印度群岛之前，它已经出现在世界许多国家。在加勒比海和南美洲北部的法国领土上，马提尼克岛报道了人类神经管圆线虫病的首例病例，瓜德罗普岛和法属圭亚那分别于2002年、2013年和2017年举行。为了更好地表征瓜德罗普岛的管圆线虫病，特别是它的地理起源和引进路线，我们对广州管圆线虫成虫及其中间寄主Achatinafulica进行了分子表征。
方法：通过聚合酶链反应（PCR）提取并扩增成年广州管圆线虫和Achatinafulica的基因组DNA，这些基因靶向线粒体基因细胞色素B和C，以及16S核糖体RNA。对PCR产物进行测序并通过系统发育分析进行研究。
结果：细胞色素B和细胞色素C分子标记表明瓜德罗普岛的A.cantonensis成虫的单系谱系。鉴定了两个黄曲霉的序列。
结论：这些结果证实了最近将广州管圆线虫和Achatinafulica引入瓜德罗普岛。瓜德罗普岛的Achatinafulica与巴巴多斯和新喀里多尼亚有着共同的起源，而瓜德罗普岛的广州管圆线虫与巴西的管圆线虫有着共同的起源，夏威夷和日本
方法：通过聚合酶链反应（PCR）提取并扩增成年广州管圆线虫和Achatinafulica的基因组DNA，这些基因靶向线粒体基因细胞色素B和C，以及16S核糖体RNA。对PCR产物进行测序并通过系统发育分析进行研究。
结果：细胞色素B和细胞色素C分子标记表明瓜德罗普岛的A.cantonensis成虫的单系谱系。鉴定了两个黄曲霉的序列。
结论：这些结果证实了最近将广州管圆线虫和Achatinafulica引入瓜德罗普岛。瓜德罗普岛的Achatinafulica与巴巴多斯和新喀里多尼亚有着共同的起源，而瓜德罗普岛的广州管圆线虫与巴西的管圆线虫有着共同的起源，夏威夷和日本
