Abstract：
Nuclear pore complexes (NPCs) are sophisticated multiprotein assemblies embedded within the nuclear envelope and controlling the exchanges of molecules between the cytoplasm and the nucleus. In this review, we summarize the mechanisms by which these elaborate complexes are built from their subunits, the nucleoporins, based on our ever-growing knowledge of NPC structural organization and on the recent identification of additional features of this process. We present the constraints faced during the production of nucleoporins, their gathering into oligomeric complexes, and the formation of NPCs within nuclear envelopes, and review the cellular strategies at play, from co-translational assembly to the enrolment of a panel of cofactors. Remarkably, the study of NPCs can inform our perception of the biogenesis of multiprotein complexes in general - and vice versa.
摘要：
核孔复合物（NPC）是嵌入核膜内并控制细胞质和细胞核之间分子交换的复杂多蛋白组装体。在这次审查中,我们总结了这些复杂的复合物从其亚基构建的机制，核孔蛋白，基于我们对NPC结构组织的不断增长的知识以及对该过程的其他特征的最新识别。我们介绍了核孔蛋白生产过程中面临的制约因素，它们聚集成寡聚复合物，以及在核包膜内形成NPC，并回顾起作用的细胞策略，从共同翻译组装到一组辅因子的注册。值得注意的是,对NPCs的研究可以使我们对一般多蛋白复合物的生物发生的看法-反之亦然。
